Salivary cortisol level and uncooperative behavior in pediatric dental practice
| Autor: | Margarete C. Ribeiro-Dasilva, Rosana de Fátima Possobon, Livia Fernandes Probst, Scott L. Tomar, Ludmila Tavares Costa Ercolin, Gláucia Maria Bovi Ambrosano |
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| Rok vydání: | 2016 |
| Předmět: |
Dental practice
Rabbit Antibody Saliva business.industry Dentistry RK1-715 030206 dentistry Dental care Dental anxiety. Salivary elimination. Dental care 03 medical and health sciences 0302 clinical medicine Medicine Cooperative behavior Analysis of variance business General Dentistry Cortisol level 030217 neurology & neurosurgery Salivary cortisol |
| Zdroj: | Brazilian Journal of Oral Sciences, Vol 15, Iss 1 (2016) |
| ISSN: | 1677-3225 |
| DOI: | 10.20396/bjos.v15i1.8647125 |
| Popis: | Aim: To investigate the relation between uncooperative behavior and salivary cortisol level in children who underwent preventive dental care. Methods: The sample was composed by 10 children of both sexes aged 40 to 52 months, presenting uncooperative behavior during dental preventive treatments. The saliva collection was performed using a cotton wheel and an Eppendorf tube (Sarstedt Salivete®) in 3 different moments: a) at home, on a day without dental treatment and at the same time on the day of the sessions treatment; b) 30 min after the end of the session, when there was manifestation of uncooperative behavior; c) 30 min after the end of the session, when there was a cooperative behavior of the child. A sample of saliva was centrifuged for 5 minutes at 2400 rpm, 1 of mL of saliva was pipetted in an Eppendorf tube and stored in a freezer at -20 ° C. For the determination of the levels of salivary cortisol was used an Active® kit for cortisol enzyme immunoassay (EIA) DSL-10-67100, composed of specific rabbit antibody anti-cortisol. Data were analyzed statistically for the uncooperative behavior issued in the beginning and at the end of sessions, using the paired t test (p |
| Databáze: | OpenAIRE |
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