Age‐related decline of interferon‐gamma responses in macrophage impairs satellite cell proliferation and regeneration
| Autor: | Jian Wu, Jie Du, Bokang Qiao, Fan Zhang, Yulin Li, Naixuan Cheng, Congcong Zhang, Chang Liu |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Aging lcsh:Diseases of the musculoskeletal system Macrophage medicine.medical_treatment CD8-Positive T-Lymphocytes CXCR3 lcsh:QM1-695 Andrology Interferon-gamma Mice 03 medical and health sciences 0302 clinical medicine Muscle regeneration Physiology (medical) Myosin medicine Animals Regeneration CXCL10 Orthopedics and Sports Medicine Interferon gamma IFN‐γ Cell Proliferation Single‐cell RNA sequence business.industry Myogenesis Macrophages lcsh:Human anatomy Original Articles medicine.disease Mice Inbred C57BL 030104 developmental biology Cytokine 030220 oncology & carcinogenesis Sarcopenia Original Article lcsh:RC925-935 business CD8 medicine.drug |
| Zdroj: | Journal of Cachexia, Sarcopenia and Muscle Journal of Cachexia, Sarcopenia and Muscle, Vol 11, Iss 5, Pp 1291-1305 (2020) |
| ISSN: | 2190-6009 2190-5991 |
| Popis: | BACKGROUND Impaired muscle regeneration and increased muscle fibrosis are observed in aged muscle accompanied by progressive loss of muscle mass (sarcopenia). However, the underlying mechanism is still unclear. METHODS The differentiated expressed genes in young and aged muscles after acute injury by cardiotoxin were identified by RNA-sequence analysis. Single-cell RNA-sequence analysis was used to identify cell clusters and functions in young muscle after acute injury, and flow cytometry analysis and sorting were used to validate the function. The proliferation and differentiation functions of satellite cells were accessed by immunostaining with 5-ethynyl-2'-deoxyuridine and embryonic myosin heavy chain (eMyHC), respectively. Muscle regeneration ability was accessed by histopathological and molecular biological methods. RESULTS Gene expression patterns associated with responses to interferon-gamma (IFN-γ) (15 genes; false discovery rate |
| Databáze: | OpenAIRE |
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