The physiological potential of anammox bacteria as revealed by their core genome structure
| Autor: | Hideto Takami, Megumi Kuroiwa, Kohei Fukuhara, Ikuo Uchiyama, Yuhki Harigaya, Atsushi Toyoda, Takashi Okubo, Takuma Suzuki, Yuichi Suwa, Yuka Murakami |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
AcademicSubjects/SCI01140
Nitrite Reductases AcademicSubjects/MED00774 Genome Bacteria Anaerobic 03 medical and health sciences Ammonium Compounds Genetics Molecular Biology Gene Hydroxylamine Oxidoreductase 030304 developmental biology Comparative genomics metagenomics 0303 health sciences Bacteria biology 030306 microbiology Sequence Analysis DNA General Medicine core genome structure Nitrite reductase biology.organism_classification Anammox Candidatus Metagenome anammox physiological potential Oxidoreductases Genome Bacterial Research Article |
| Zdroj: | DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes |
| ISSN: | 1756-1663 |
| DOI: | 10.1093/dnares/dsaa028 |
| Popis: | We present here the second complete genome of anaerobic ammonium oxidation (anammox) bacterium, Candidatus (Ca.) Brocadia pituitae, along with those of a nitrite oxidizer and two incomplete denitrifiers from the anammox bacterial community (ABC) metagenome. Although NO2− reduction to NO is considered to be the first step in anammox, Ca. B. pituitae lacks nitrite reductase genes (nirK and nirS) responsible for this reaction. Comparative genomics of Ca. B. pituitae with Ca. Kuenenia stuttgartiensis and six other anammox bacteria with nearly complete genomes revealed that their core genome structure contains 1,152 syntenic orthologues. But nitrite reductase genes were absent from the core, whereas two other Brocadia species possess nirK and these genes were horizontally acquired from multiple lineages. In contrast, at least five paralogous hydroxylamine oxidoreductase genes containing candidate ones (hao2 and hao3) encoding another nitrite reductase were observed in the core. Indeed, these two genes were also significantly expressed in Ca. B. pituitae as in other anammox bacteria. Because many nirS and nirK genes have been detected in the ABC metagenome, Ca. B. pituitae presumably utilises not only NO supplied by the ABC members but also NO and/or NH2OH by self-production for anammox metabolism. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|