Memory Function in Feeding Habit Transformation of Mandarin Fish (Siniperca chuatsi)
| Autor: | Lin-Jie Shi, Wenjing Cai, Yaqi Dou, Jie Wang, Xu-Fang Liang, Shan He, Jiao Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
CAMP Responsive Element Binding Protein Taste media_common.quotation_subject Zoology Biology CREB Catalysis Article mandarin fish (Siniperca chuatsi) Receptors G-Protein-Coupled Inorganic Chemistry memory lcsh:Chemistry 03 medical and health sciences Habits 0302 clinical medicine feeding habit transformation training repeated training DNA methylation Siniperca chuatsi Animals Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy media_common Organic Chemistry Fishes General Medicine biology.organism_classification Computer Science Applications Transformation (genetics) 030104 developmental biology CpG site lcsh:Biology (General) lcsh:QD1-999 Forage fish biology.protein CpG Islands Habit 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences, Vol 19, Iss 4, p 1254 (2018) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 19; Issue 4; Pages: 1254 |
| ISSN: | 1422-0067 |
| Popis: | Mandarin fish refuse dead prey fish or artificial diets and can be trained to transform their inborn feeding habit. To investigate the effect of memory on feeding habit transformation, we compared the reaction time to dead prey fish and the success rate of feeding habit transformation to dead prey fish with training of mandarin fish in the 1st experimental group (trained once) and the 2nd experimental group (trained twice). The mandarin fish in the 2nd group had higher success rate of feeding habit transformation (100%) than those in the 1st group (67%), and shorter reaction time to dead prey fish (1 s). Gene expression of cAMP responsive element binding protein I (Creb I), brain-derived neurotrophic factor (Bdnf), CCAAT enhancer binding protein delta (C/EBPD), fos-related antigen 2 (Fra2), and proto-oncogenes c-fos (c-fos) involved in long-term memory formation were significantly increased in the 2nd group after repeated training, and taste 1 receptor member 1 (T1R1), involved in feeding habit formation, was significantly increased in brains of the 2nd group after repeated training. DNA methylation levels at five candidate CpG (cytosine–guanine) sites contained in the predicted CpG island in the 5′-flanking region of T1R1 were significantly decreased in brains of the 2nd group compared with that of the 1st group. These results indicated that the repeated training can improve the feeding habit transformation through the memory formation of accepting dead prey fish. DNA methylation of the T1R1 might be a regulatory factor for feeding habit transformation from live prey fish to dead prey fish in mandarin fish. |
| Databáze: | OpenAIRE |
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