Isolation and multipotential differentiation of mesenchymal stromal cell‑like progenitor cells from human bladder
| Autor: | Jun Lu, Hui‑Yue Dong, Ling‑Feng Zhu, Yuan‑Ming Cai, Jian‑Ming Tan, Ling Zhu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Cell type Telomerase Stromal cell Cell Myocytes Smooth Muscle Urinary Bladder Biology Biochemistry Umbilical Cord 03 medical and health sciences Tissue engineering Osteogenesis Genetics medicine Myocyte Humans Cell Lineage Endothelium Progenitor cell Molecular Biology bladder Cells Cultured Aged Adipogenesis Tissue Engineering Stem Cells Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Articles differentiation Middle Aged 030104 developmental biology medicine.anatomical_structure Oncology Cancer research Molecular Medicine Female Urothelium mesenchymal stromal cells Chondrogenesis |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | Various types of mesenchymal stromal cells (MSCs) have been used in urological tissue engineering but to date the existence of MSCs has not been reported in the human bladder. The present study provided evidence that a small number of MSC-like cells exist in the human bladder and designated this class of cells ‘human bladder-derived MSC-like cells’ (hBSCs). It was demonstrated that hBSCs can be cultured to yield a large population. These hBSCs expressed the surface markers of MSCs and exhibited the capacity for osteogenic, adipogenic and chondrogenic differentiation. On induction with appropriate media in vitro, hBSCs could differentiate into bladder-associated cell types, including urothelial, endothelial and smooth muscle cell-like lineages. In addition, the average telomerase activity of adult hBSCs was higher compared with adult human bone marrow-derived MSCs, but lower than that of human umbilical cord Wharton's jelly-derived MSCs. These findings may inspire future studies on the role of hBSCs in urological tissue engineering applications and in other fields. |
| Databáze: | OpenAIRE |
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