A soft anti-virulence liposome realizing the explosive release of antibiotics at an infectious site to improve antimicrobial therapy
| Autor: | Mengyuan Liu, Jingyi An, Shizhen Geng, Xiang Lu, Ge Zhang, Lulu Sun, Zhenzhong Zhang, Hongling Zhang, Shudong Zhang, Binghua Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Staphylococcus aureus
medicine.drug_class Antibiotics Biomedical Engineering Phospholipid Virulence 02 engineering and technology Microbiology Mice 03 medical and health sciences chemistry.chemical_compound Anti-Infective Agents Explosive Agents Phosphatidylcholine Human Umbilical Vein Endothelial Cells medicine Animals Humans General Materials Science 030304 developmental biology 0303 health sciences Liposome Chemistry General Chemistry General Medicine 021001 nanoscience & nanotechnology Antimicrobial Anti-Bacterial Agents Sphingomyelins Cholesterol Liposomes Nanoparticles Vancomycin Female Staphylococcal Skin Infections 0210 nano-technology Sphingomyelin medicine.drug |
| Zdroj: | Journal of Materials Chemistry B. 9:147-158 |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/d0tb02255a |
| Popis: | Pore-forming toxins (PFTs), the most common virulence proteins, are promising therapeutic keys in bacterial infections. CAL02, consisting of sphingomyelin (Sm) and containing a maximum ratio of cholesterol (Ch), has been applied to sequester PFTs. However, Sm, a saturated phospholipid, leads to structural rigidity of the liposome, which does not benefit PFT combination. Therefore, in order to decrease the membrane rigidity and improve the fluidity of liposomes, we have introduced an unsaturated phospholipid, phosphatidylcholine (Pc), to the saturated Sm. In this report, a soft nanoliposome (called CSPL), composed of Ch, Sm and Pc, was artificially prepared. In order to further improve its antibacterial effect, vancomycin (Van) was loaded into the hydrophilic core of CSPL, where Van can be released radically at the infectious site through transmembrane pores formed by the PFTs in CSPL. This soft Van@CSPL nanoliposome with detoxification/drug release was able to inhibit the possibility of antibiotic resistance and could play a better role in treating severe invasive infections in mice. |
| Databáze: | OpenAIRE |
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