Alteration of Tubulin-Gi Protein Interaction in Rat Cerebral Cortex with Aging
| Autor: | Toshikazu Saito, Hiroki Ozawa, Hideyo Ohshika, Shinichi Hatta |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Aging Azides GTP' Macromolecular Substances Immunoblotting Gi alpha subunit Biochemistry ADCY10 Adenylyl cyclase Cellular and Molecular Neuroscience chemistry.chemical_compound GTP-Binding Proteins Tubulin Microtubule medicine Animals Rats Wistar Cerebral Cortex Guanylyl Imidodiphosphate biology Cell Membrane ADCY9 Affinity Labels Molecular biology Rats medicine.anatomical_structure chemistry Cerebral cortex Adenylyl Cyclase Inhibitors biology.protein Guanosine Triphosphate |
| Zdroj: | Journal of Neurochemistry. 63:1104-1110 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1046/j.1471-4159.1994.63031104.x |
| Popis: | The ability of the tubulin dimer to interact with and to modulate the Gi function inhibiting adenylyl cyclase was examined in cerebral cortex membranes from 2-month-old and 24-month-old rats. The hydrolysis-resistant GTP analogue 5'-guanylylimidodiphosphate (GppNHp)-dependent inhibition of adenylyl cyclase was significantly decreased in cerebral cortex membranes from 24-month-old rats. Tubulin, prepared from rat brains by polymerization with GppNHp, caused inhibition of adenylyl cyclase (approximately 28%) in 2-month-old rats. Tubulin-GppNHp-dependent inhibition of adenylyl cyclase in 24-month-old rats was significantly attenuated (approximately 15%). In 2-month-old rats, when tubulin, polymerized with the hydrolysis-resistant photoaffinity GTP analogue [32P]P3(4-azidoanilido)-P1-5'-GTP ([32P]AAGTP), was incubated with cerebral cortex membranes, AAGTP was transferred from tubulin to Gi alpha. Transfer of AAGTP from tubulin to Gi alpha was reduced in 24-month-old rats. Furthermore, photoaffinity labeling of [32P]AAGTP to Gi alpha in cortex membranes was significantly decreased in 24-month-old rats. No differences were observed in the amounts of Gs alpha, Gi alpha, or G beta subunits and tubulin, estimated by immunoblotting, in cortex membranes from 2-month-old and 24-month-old rats. These results suggest that the ability of tubulin to interact with Gi and thereby modulate the inhibitory regulation of adenylyl cyclase is reduced in the cerebral cortex of 24-month-old rats. |
| Databáze: | OpenAIRE |
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