Increased expression of tissue factor mRNA and procoagulant activity in ovine lentivirus-infected alveolar macrophages
| Autor: | J. Belleville, T. Greenland, A.-M. Freyria, G. Cordier, Jean-Luc Cadoré, M. Lyon, Jean-François Mornex, François Guiguen, P. Lena |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary Visna-maedi virus Visna virus viruses Immunology Molecular Sequence Data Gene Expression Inflammation In Vitro Techniques Thromboplastin Tissue factor In vivo Virology Macrophages Alveolar medicine Macrophage Animals Northern blot RNA Messenger DNA Primers Lung Sheep biology Base Sequence Pneumonia Progressive Interstitial of Sheep respiratory system biology.organism_classification Blood Coagulation Factors medicine.anatomical_structure Pulmonary alveolus medicine.symptom |
| Zdroj: | Research in virology. 145(3-4) |
| ISSN: | 0923-2516 |
| Popis: | Summary To link ovine lentivirus infection to lung tissue damage, we studied the procoagulant response in alveolar macrophages from experimentally infected lambs and in in vitro infected alveolar macrophages. We cloned ovine tissue factor cDNA and analysed its in vitro expression by Northern blotting. Visna-maedi virus induced tissue factor mRNA. In order to correlate this mRNA induction with its cellular function, we analysed macrophage procoagulant activity after in vitro and in vivo infection. The procoagulant activity was increased by interaction with the virus in both cases. Thus, visna-maedi virus-induced expression of tissue factor mRNA was associated with enhanced macrophage procoagulant activity. These findings indicate an active role of alveolar macrophages in the pathogenesis of these inflammatory lung lesions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|