Detection of Kingella kingae Osteoarticular Infections in Children by Oropharyngeal Swab PCR
| Autor: | Léopold Lamah, Christophe Combescure, Sergio Manzano, Victor Dubois-Ferriere, Dimitri Ceroni, Abdessalam Cherkaoui, Jacques Schrenzel, Renzi Gesuele, Jonathan Hibbs |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cartilage
Articular Male musculoskeletal diseases Oropharyngeal swab Polymerase Chain Reaction/methods Neisseriaceae Infections Cartilage Articular/microbiology/pathology Oropharynx/microbiology Specimen Handling/methods Pcr assay Oropharynx Kingella kingae Neisseriaceae Infections/diagnosis/microbiology Polymerase Chain Reaction Specimen Handling law.invention Microbiology Predictive Value of Tests law Humans Medicine Prospective Studies Polymerase chain reaction ddc:616 Oropharyngeal disorders ddc:618 biology business.industry Infant Kingella kingae/isolation & purification/pathogenicity Osteomyelitis Osteomyelitis/diagnosis/genetics/microbiology biology.organism_classification Child Preschool Predictive value of tests Pediatrics Perinatology and Child Health Female business Clinical evaluation |
| Zdroj: | Pediatrics, Vol. 131, No 1 (2013) pp. e230-5 |
| ISSN: | 1098-4275 0031-4005 |
| DOI: | 10.1542/peds.2012-0810 |
| Popis: | OBJECTIVE: The purpose of this study was to investigate if oropharyngeal swab polymerase chain reaction (PCR) could predict osteoarticular infection (OAI) due to Kingella kingae in young children. METHODS: One hundred twenty-three consecutive children aged 6 to 48 months presenting with atraumatic osteoarticular complaints were prospectively studied. All had a clinical evaluation, imaging, and blood samples. Blood and oropharyngeal specimens were tested with a PCR assay specific for K kingae. OAI was defined as bone, joint, or blood detection of pathogenic bacteria, or MRI consistent with infection in the absence of positive microbiology. K kingae OAI was defined by blood, bone, or synovial fluid positivity for the organism by culture or PCR. RESULTS: Forty children met the OAI case definition; 30 had K kingae OAI, 1 had another organism, and 9 had no microbiologic diagnosis. All 30 oropharyngeal swabs from the K kingae case patients and 8 swabs from the 84 patients without OAI or with OAI caused by another organism were positive. The sensitivity and specificity of the oropharyngeal swab PCR assay for K kingae were 100% and 90.5%, respectively. CONCLUSIONS: Detection of K kingae DNA in oropharyngeal swabs of children with clinical findings of OAI is predictive of K kingae OAI. If these findings are replicated in other settings, detection of K kingae by oropharyngeal swab PCR could improve the recognition of OAI. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|