Nonomuraea purpurea sp. nov., an actinomycete isolated from mangrove sediment
| Autor: | Kannika Duangmal, Yoko Takahashi, Ratchanee Mingma, Nantana Srisuk, Atsuko Matsumoto, Paweena Suksaard |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial Geologic Sediments Diamino acid Peptidoglycan Biology Rhizophila Diaminopimelic Acid Microbiology 03 medical and health sciences chemistry.chemical_compound Phylogenetics Cell Wall RNA Ribosomal 16S Botany Ecology Evolution Behavior and Systematics Mycelium Phospholipids Phylogeny Soil Microbiology Base Composition Strain (chemistry) Fatty Acids Nucleic Acid Hybridization Vitamin K 2 General Medicine Sequence Analysis DNA Ribosomal RNA 16S ribosomal RNA biology.organism_classification Thailand Bacterial Typing Techniques Actinobacteria 030104 developmental biology chemistry Wetlands Avicennia |
| Zdroj: | International journal of systematic and evolutionary microbiology. 66(12) |
| ISSN: | 1466-5034 |
| Popis: | A polyphasic approach was used to verify the novel actinomycete, strain 1SM4-01T, isolated from mangrove sediment collected from Ranong Province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism was a member of the genus Nonomuraea and was most closely related to Nonomuraea syzygii GKU 164T (98.7 % sequence similarity), Nonomuraea rhizophila YIM 67092T (98.4 %), Nonomuraea solani NEAU-Z6T (98.4 %), Nonomuraea monospora PT708T (98.3 %) and Nonomuraea thailandensis KC-061T (98.2 %). The strain produced branching aerial mycelium which differentiated into straight chains of rough-surfaced spores borne at the end of a short sporophore. The whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, with madurose, mannose and ribose as the main sugars. MK-9(H4) was a major menaquinone of this strain. The acyl type of peptidoglycan was N-acetyl. The predominant cellular fatty acids were C17 : 1 ω8c and iso-C16 : 0. Phospholipids consisted of diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, aminophospholipids and unidentified lipids. The G+C content of the genomic DNA was 70.4 mol%. On the basis of phenotypic characteristics, DNA–DNA relatedness and phylogenetic distinctiveness, strain 1SM4-01T represents a novel species of the genus Nonomuraea , for which the name Nonomuraea purpurea sp. nov. is proposed. The type strain is 1SM4-01T (=BCC 60397T=NBRC 109647T). |
| Databáze: | OpenAIRE |
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