Contribution of the major and minor subunits to fimbria-mediated adherence of Haemophilus influenzae to human epithelial cells and erythrocytes
| Autor: | F. R. Mooi, J. P. M. Van Putten, L. Van Alphen, S. M. Van Ham |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Adult
Erythrocytes Protein subunit Immunology Fimbria Oropharynx medicine.disease_cause Microbiology Pilus Bacterial Adhesion Epithelium Haemophilus influenzae Fimbriae Proteins Antigen Bacterial Proteins medicine Humans Escherichia coli biology Pasteurellaceae virus diseases biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses Infectious Diseases Fimbriae Bacterial Multigene Family Mutation bacteria Parasitology Research Article |
| Zdroj: | Infection and immunity, 63(12), 4883-4889. American Society for Microbiology |
| ISSN: | 0019-9567 |
| Popis: | Fimbriae are colonization factors of the human pathogen Haemophilus influenzae in that they mediate bacterial adherence to human eukaryotic cells. The contribution of the major (HifA) and putative minor (HifD and HifE) subunits of H. influenzae fimbriae to fimbria-specific adherence was studied by using mutants that were inactivated in distinct fimbrial genes. Both the major and minor subunits were required for adherence of H. influenzae to oropharyngeal epithelial cells and human erythrocytes carrying the AnWj antigen. Cloning of defined H. influenzae fimbrial genes in an Escherichia coli strain with type 1 fimbriae yielded recombinants expressing high amounts of HifA-containing H. influenzae fimbriae either with or without coexpression of both H. influenzae minor subunits. Both clones exhibited the specific adherence properties of H. influenzae fimbriae, implying that the minor H. influenzae subunits are dispensable for adherence and that the adhesive domain resides in the major subunit, HifA. In H. influenzae itself, the minor subunits probably affect adherence by raising the number of fimbriae above the minimal level required to establish adherence. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|