Genetic Diversity in Diospyros Germplasm in the Western Caucasus Based on SSR and ISSR Polymorphism
| Autor: | Ruslan S Rakhmangulov, Magomed Dzhamaludinovich Omarov, Lidia Samarina, R. V. Kulyan, Natalia G. Koninskaya, Alexey V. Ryndin, Ekaterina S. Shurkina, Valentina Malyarovskaya, Gregory Agasievich Tsaturyan, Alexandra O. Matskiv, Ruset M. Shkhalakhova, Zuhra M. Omarova, Stefanie Reim |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm microsatellite QH301-705.5 Lotus ISSR-marker 01 natural sciences Article General Biochemistry Genetics and Molecular Biology polymorphism 03 medical and health sciences Botany Genotype Cultivar Biology (General) Genotyping Genetic diversity General Immunology and Microbiology biology Diospyros genetic diversity biology.organism_classification amplified fragment persimmon 030104 developmental biology genotyping Microsatellite General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Biology, Vol 10, Iss 341, p 341 (2021) Biology Volume 10 Issue 4 |
| ISSN: | 2079-7737 |
| Popis: | Simple Summary Persimmon is an edible fruit consisting of several species in the genus Diospyros. The most widely cultivated and most commercially important species is the Oriental persimmon, Diospyros kaki. However, the inter- and intra-specific genetic diversity of the genus Diospyros remains largely unclear and is of great interest both for conservation and breeding purposes. This study describes the genetic diversity and genetic admixture of Diospyros germplasm in the Western Caucasus. The information can be used to support conservation measures and the breeding of persimmon. Abstract Persimmon germplasm in the Western Caucasus represent one of the most northerly collections. In our study, 51 commercial cultivars of D. kaki, 3 accessions of D. virginiana and 57 D. lotus accessions from six geographically distant populations were investigated using 19 microsatellite and 10 inter simple sequence repeat (ISSR) markers. After STRUCTURE analysis, the single accessions of Diospyros were allocated to three genetic clusters. Genetic admixtures in the important genotypes of D. kaki were revealed, whereas D. lotus accessions showed no admixture with other genetic clusters. The correspondence of genetic data and phenotypical traits was estimated in the D. kaki collection. The most frost tolerant genotypes of the collection, such as “Mountain Rogers”, “Nikitskaya Bordovaya”, “Rossiyanka”, “MVG Omarova”, “Meader”, “Costata”, “BBG”, and “Jiro”, showed a high percentage of genetic admixtures and were grouped close to D. virginiana. Some of these genotypes are known to be interspecific hybrids with D. virginiana. A low level of genetic diversity between the distant D. lotus populations was revealed and it can be speculated that D. lotus was introduced to the Western Caucasus from a single germplasm source. These results are an important basis for the implementation of conservation measures, developing breeding strategies, and improving breeding efficiency. |
| Databáze: | OpenAIRE |
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