Molecular characterization of a Brugia malayi transglutaminase
| Autor: | Eswaran Devarajan, Kaliraj Perumal, Shiva Thirugnanam, Pankaj K. Mishra, Ramaswamy Chandrashekar, Kapil Mehta |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Complementary Tissue transglutaminase Molecular Sequence Data Antibodies Helminth Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Brugia malayi law.invention law Complementary DNA parasitic diseases Animals Humans Amino Acid Sequence Cloning Molecular Protein disulfide-isomerase Messenger RNA Transglutaminases General Veterinary biology Molecular mass Reverse Transcriptase Polymerase Chain Reaction Endoplasmic reticulum Sequence Analysis DNA General Medicine biology.organism_classification Molecular biology Recombinant Proteins Filariasis Infectious Diseases Insect Science Recombinant DNA biology.protein Female Parasitology |
| Zdroj: | Parasitology Research. 93:145-150 |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Prior studies have demonstrated that transglutaminase (TGase) from the human filarial parasite Brugia malayi is critical for the growth and development of the larval stages. In this report, we describe the cloning and partial characterization of a cDNA encoding the B. malayi TGase (BmTGase). Using RT-PCR and RACE-PCR, the cDNA was amplified from adult worm mRNA. BmTGase is 1,881 bp long and codes for a protein with a predicted molecular mass of 54 kDa. Amino acid sequence analysis of BmTGase revealed significant homology to the protein disulfide isomerase (PDI), particularly, to the PDI-related protein ERp60, a PDI isoform found in the lumen of endoplasmic reticulum. The activity of recombinant B. malayi TGase enzyme (rBmTG) was found to be calcium-dependent and could be inhibited by EDTA. ELISA studies showed that approximately 88% of 48 sera from healthy Indian patients living in a bancroftian filariasis endemic area were reactive with rBmTG. In contrast, only 33% of sera from patients with clinical filariasis were reactive to rBmTG. Non-endemic sera were uniformly non-reactive. Additional studies are needed to elucidate the role, if any, of B. malayi TGase in protective immunity to filariasis. |
| Databáze: | OpenAIRE |
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