Characterization of E. coli Isolates Producing Extended Spectrum Beta-Lactamase SHV-Variants from the Food Chain in Germany
| Autor: | Katharina Juraschek, Alexandra Irrgang, Annemarie Kaesbohrer, Ge Zhao, Jens A. Hammerl |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Transposable element Tn3 transposon SHV-12 QH301-705.5 medicine.medical_treatment Integron Microbiology 03 medical and health sciences Plasmid Virology IncX3 Pulsed-field gel electrophoresis medicine polycyclic compounds Biology (General) 030304 developmental biology 2. Zero hunger Genetics 0303 health sciences biology 030306 microbiology biochemical phenomena metabolism and nutrition bacterial infections and mycoses Composite transposon ESBL food chain Horizontal gene transfer biology.protein Beta-lactamase bacteria IncI1 SHV-2 |
| Zdroj: | Microorganisms, Vol 9, Iss 1926, p 1926 (2021) Microorganisms Volume 9 Issue 9 |
| ISSN: | 2076-2607 |
| Popis: | Resistance of bacteria to 3rd generation cephalosporins mediated by beta-lactamases (ESBL, pAmpC) is a public health concern. In this study, 1517 phenotypically cephalosporin-resistant E. coli were screened for the presence of blaSHV genes. Respective genes were detected in 161 isolates. Majority (91%) were obtained from poultry production and meat. The SHV-12 beta-lactamase was the predominant variant (n = 155), while the remaining isolates exhibited SHV-2 (n = 4) or SHV-2a (n = 2). A subset of the isolates (n = 51) was further characterized by PCR, PFGE, or whole-genome sequencing and bioinformatics analysis. The SHV-12-producing isolates showed low phylogenetic relationships, and dissemination of the blaSHV-12 genes seemed to be mainly driven by horizontal gene transfer. In most of the isolates, blaSHV-12 was located on transferable IncX3 (~43 kb) or IncI1 (~100 kb) plasmids. On IncX3, blaSHV-12 was part of a Tn6 composite transposon located next to a Tn3 transposon, which harbored the fluoroquinolone resistance gene qnrS1. On IncI1 plasmids, blaSHV-12 was located on an incomplete class 1 integron as part of a Tn21 transposon. In conclusion, SHV-12 is widely distributed in German poultry production and spreads via horizontal gene transfer. Consumers are at risk by handling raw poultry meat and should take care in appropriate kitchen hygiene. |
| Databáze: | OpenAIRE |
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