Nitrergic Response TO Cyclophosphamide Treatment in Blood and Bone Marrow
| Autor: | R.M Ohanyan, H. L. Hayrapetyan, N. Kh. Alchujyan, G. A. Kevorkian, S.S Dagbashyan, N. H. Movsesyan, A. G. Guevorkian |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Lymphocyte medicine.medical_treatment Intraperitoneal injection arginine Article General Biochemistry Genetics and Molecular Biology Nitric oxide chemistry.chemical_compound Internal medicine medicine rat Platelet Cyclophosphamide Reactive nitrogen species biology nitric oxide synthase business.industry Monocyte marrow Nitric oxide synthase Endocrinology medicine.anatomical_structure chemistry citrulline biology.protein Bone marrow blood formed elements business |
| Zdroj: | The Open Biochemistry Journal |
| ISSN: | 1874-091X |
| DOI: | 10.2174/1874091x00802010081 |
| Popis: | Daily intraperitoneal injection of cyclophosphamide (CPA) (50 mgkg(-1) of body weight) for 5 days resulted in reduced levels of marrow and blood cellularity, which was most pronounced in 18 days post-treatment (pt). On day 18 after CPA treatment the enhancedlevels of nitric oxide (NO) precursors and metabolites (L-arginine, L-citrulline, reactive nitrogen species (RNS)) of marrow and blood cells (platelet, neutrophil, lymphocyte and monocyte) resulted from up-regulation of Ca(II)/calmodulin(CaM)-independent "inducible" NO synthase (iNOS), with a lessercontribution of Ca(II)/CaM-dependent "constitutive" cNOS isoforms to systemic NO.Biphasic response to CPA of marrow nitrergic system, i.e. both iNOS and cNOS showed significantly depressed activities, as well as diminished levels of NO metabolites on day 9 pt, suggested that signals in addition to NO might be involved in CPA-induced inhibition of hematopoesis, while a gradual increase of neutrophil and platelet NOS activity appeared to be contributed to a CPA-induced development of granulopenia, thrombocytopenia and hemorrhage. |
| Databáze: | OpenAIRE |
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