Pulsed electromagnetic fields promote repair of focal articular cartilage defects with engineered osteochondral constructs
| Autor: | J. Chloë Bulinski, Aaron M. Stoker, Robert M. Stefani, Clark T. Hung, Gordana Vunjak-Novakovic, James L. Cook, Andrea R. Tan, Sofia Barbosa, Roy K. Aaron, Gerard A. Ateshian, Ruggero Cadossi, Stefania Setti |
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| Rok vydání: | 2020 |
| Předmět: |
Cartilage
Articular Male 0106 biological sciences 0301 basic medicine Bioengineering Articular cartilage 01 natural sciences Applied Microbiology and Biotechnology Article Chondrocyte Glycosaminoglycan 03 medical and health sciences Chondrocytes Dogs Electromagnetic Fields In vivo 010608 biotechnology medicine Animals Cells Cultured Wound Healing Tissue Engineering biology business.industry Cartilage Articular cartilage injuries medicine.disease Stifle 030104 developmental biology medicine.anatomical_structure Proteoglycan Joint pain biology.protein medicine.symptom business Biotechnology Biomedical engineering |
| Zdroj: | Biotechnol Bioeng |
| ISSN: | 1097-0290 0006-3592 |
| Popis: | Articular cartilage injuries are a common source of joint pain and dysfunction. We hypothesized that pulsed electromagnetic fields (PEMFs) would improve growth and healing of tissue-engineered cartilage grafts in a direction-dependent manner. PEMF stimulation of engineered cartilage constructs was first evaluated in vitro using passaged adult canine chondrocytes embedded in an agarose hydrogel scaffold. PEMF coils oriented parallel to the articular surface induced superior repair stiffness compared to both perpendicular PEMF (p = .026) and control (p = .012). This was correlated with increased glycosaminoglycan deposition in both parallel and perpendicular PEMF orientations compared to control (p = .010 and .028, respectively). Following in vitro optimization, the potential clinical translation of PEMF was evaluated in a preliminary in vivo preclinical adult canine model. Engineered osteochondral constructs (∅ 6 mm × 6 mm thick, devitalized bone base) were cultured to maturity and implanted into focal defects created in the stifle (knee) joint. To assess expedited early repair, animals were assessed after a 3-month recovery period, with microfracture repairs serving as an additional clinical control. In vivo, PEMF led to a greater likelihood of normal chondrocyte (odds ratio [OR]: 2.5, p = .051) and proteoglycan (OR: 5.0, p = .013) histological scores in engineered constructs. Interestingly, engineered constructs outperformed microfracture in clinical scoring, regardless of PEMF treatment (p < .05). Overall, the studies provided evidence that PEMF stimulation enhanced engineered cartilage growth and repair, demonstrating a potential low-cost, low-risk, noninvasive treatment modality for expediting early cartilage repair. |
| Databáze: | OpenAIRE |
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