The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E
| Autor: | Peter Coyle, Gheyath K. Nasrallah, Enas S. Al Absi, Makiyeh Khalili, Sara K. Abdelghany, Nader Al-Dewik, Nadin Younes, Hadi M. Yassine, Somaia S. Abouzid, Asma A. Al Thani, Duaa W. Al-Sadeq |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty viruses 030106 microbiology RT-PCR India RC799-869 Sensitivity and Specificity Virus Serology 03 medical and health sciences Germany Internal medicine Hepatitis E virus Prevalence TaqMan Humans Medicine Pakistan Non-A-C hepatitis Qatar Hepatitis Wantai ELISA business.industry Gastroenterology virus diseases General Medicine Diseases of the digestive system. Gastroenterology Hepatology medicine.disease Hepatitis E Virology digestive system diseases Indian subcontinent 030104 developmental biology Immunoglobulin M Immunoglobulin G Test performance RNA Viral business Viral load Research Article |
| Zdroj: | BMC Gastroenterology, Vol 21, Iss 1, Pp 1-11 (2021) BMC Gastroenterology |
| ISSN: | 1471-230X |
| DOI: | 10.1186/s12876-021-01841-2 |
| Popis: | Background The rapid growth of Qatar in the last two decades has attracted a large influx of immigrant workers who mostly come from HEV-hyperendemic countries. Thus, we aim to investigate the prevalence of HEV among acute non-A-C hepatitis patients in Qatar; and to evaluate the performance of four dominant commercial serological assays for HEV diagnosis. Methods 259 patients with non-A-C hepatitis were tested using the Wantai HEV-IgM, HEV-IgG, HEV-Ag ELISA kits, and the MP Biomedical HEV-Total Ab ELISA kit. ALT levels were tested and HEV RNA (viral loads) was performed using Taqman AmpliCube HEV RT-PCR kit (Mikrogen, Neuried, Germany). The performance of each kit was assessed according to the RT-PCR results. Results HEV-RNA was detected in 23.1% of the samples. Most of these HEV-RNA-positive cases belonged to non-Qatari residents from the Indian subcontinent; India, Pakistan, etc. HEV-Ag, HEV-IgM, HEV-IgG, HEV-Total Ab were detected in 5.56%, 8.65%, 32.1%, and 34.2% of all tested samples, respectively. Elevated ALT levels were highly correlated with the HEV-Ag, HEV-IgM, HEV-RNA but not with the HEV-IgG and HEV-Total Ab. Although HEV-Ag was very specific (100%), yet its sensitivity was poor (36.7%). HEV-IgM demonstrated the best second marker for diagnosis of acute HEV after RT-PCR as jugged by the overall performance parameters: specificity (96.2%), sensitivity (71.4%), PPV (83.3%), NPP (92.7%), agreement with RT-PCR (91.0%), and Kappa-value (0.71). Conclusion Our study demonstrated a high prevalence of HEV virus in Qatar, mostly among immigrants from the Indian subcontinent. The HEV-IgM represents the best marker for detecting the acute HEV infection, where RT-PCR cannot be performed. |
| Databáze: | OpenAIRE |
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