Human papillomavirus infection in females with normal cervical cytology: Genotyping and phylogenetic analysis among women in Punjab, Pakistan

Autor: Mohammad Faheem, Nuzhat Rasheed, Hafsa Aziz, Huma Iqbal, Sanum Napper, Humera Mahmood, Shazia Fatima, Areej Abdul Sattar, S. S. Batool, Sobia Tabassum
Rok vydání: 2017
Předmět:
0301 basic medicine
Cervix Uteri
Iran
0302 clinical medicine
Risk Factors
Genotype
Epidemiology
Prevalence
Mass Screening
Pakistan
Papillomaviridae
Phylogeny
Cervical cancer
Aged
80 and over
education.field_of_study
Human papillomavirus 16
Phylogenetic analysis
Phylogenetic tree
Human papillomavirus 18
virus diseases
General Medicine
Middle Aged
female genital diseases and pregnancy complications
Infectious Diseases
medicine.anatomical_structure
030220 oncology & carcinogenesis
Female
Microbiology (medical)
Adult
medicine.medical_specialty
Human papillomavirus
HPV
Population
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Young Adult
medicine
Humans
HR-HPV
lcsh:RC109-216
education
Genotyping
Cervix
Aged
Gynecology
business.industry
Papillomavirus Infections
Cancer
medicine.disease
LR-HPV
030104 developmental biology
business
Demography
Zdroj: International Journal of Infectious Diseases, Vol 66, Iss C, Pp 83-89 (2018)
ISSN: 1878-3511
Popis: Background Globally, cervical cancer is the fourth most common cancer in women and the seventh most common cancer overall, accounting for an estimated 300 000 annual deaths. Human papillomavirus (HPV) is the second most common cause of cervical cancer worldwide. HPV screening is not a common practice in Pakistan. The aim of this study was to determine the prevalence of HPV and HPV types in women with a normal cytology of the cervix living in the upper and lower regions of Punjab, Pakistan, and to analyze the risk factors for HPV in this region. Methods PCR analysis was performed for 1011 female patients with a normal cytology of the cervix from various districts of Punjab Province, Pakistan. Risk factors for the acquisition of HPV were studied. High-risk HPV types (HPV16 and HPV18) were detected using the Abbott Real Time HR HPV test. To determine the genotype, partial L1 region sequences of HPV-positive samples were subjected to sequencing using MY/09/MY11 primers, and a phylogenetic tree was constructed using CLC software. Results The study found a 4.74% prevalence of HPV, with the most frequent HPV type found being the low-risk HPV6 (in 25% of infected individuals), followed by HPV55 (22.9%), HPV11 (20.8%), and high-risk types HPV45 (12.5%), HPV33 (8.33%), HPV18 (6.25%), and HPV16 (4.16%). Phylogenetic analysis of all HPV types in this study showed 80–99% nucleotide identity with types related to the same species. The sequences were clustered with China, India, Mexico, Iran, Slovenia, and Germany, showing the diversity in origin of the various genotypes prevalent in Pakistan. Conclusions In this population with a normal cervical cytology, the prevalence of high-risk HPV types was very low. The major prevalent HPV genotype in Punjab Province of Pakistan was the low-risk HPV type 6, followed by HPV type 55. Sequencing of the partial L1 region suggested that the region was highly conserved in all reported sequences. This study highlights the need to conduct robust epidemiological studies in the region and to develop regular HPV screening so that the situation does not reach an alarming stage resulting in cervical cancer.
Databáze: OpenAIRE
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