Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
| Autor: | Marcela Manduca Ferreira, Hélio José Montassier, T.S. Martins, Mariela Aparecida Claro Martines, Beatriz B. Zambotti, Gabriel Yuri Storino, Henrique Meiroz de Souza Almeida, Karina Sonalio, Márcia Carvalho de Abreu Fantini, Fernando Antônio Moreira Petri, Osvaldo A. Sant'Anna, Luís Guilherme de Oliveira, Marina Lopes Mechler-Dreibi |
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| Přispěvatelé: | Universidade Estadual Paulista (UNESP), Universidade de São Paulo (USP), Butantan Institute |
| Rok vydání: | 2021 |
| Předmět: |
Swine
medicine.medical_treatment Science animal diseases Biopsy Administration Oral medicine.disease_cause Real-Time Polymerase Chain Reaction Article Porcine enzootic pneumonia Mycoplasma hyopneumoniae Adjuvants Immunologic Medicine Animals Adjuvants IMUNIZAÇÃO Lung Multidisciplinary biology business.industry Vaccination Mycoplasma Pneumonia of Swine Mycoplasmal biology.organism_classification Silicon Dioxide Immunohistochemistry Immunoglobulin A medicine.anatomical_structure Treatment Outcome Immunization Immunoglobulin G Immunology Bacterial Vaccines Cytokines Infectious diseases Bacterial infection business Adjuvant Bronchoalveolar Lavage Fluid Respiratory tract |
| Zdroj: | Scientific Reports Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 2045-2322 |
| Popis: | Made available in DSpace on 2022-04-28T19:47:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Mycoplasma (M.) hyopneumoniae is the main pathogen of porcine enzootic pneumonia (PEP). Its controlling is challenging, and requires alternative strategies. This study aimed to develop an oral vaccine against M. hyopneumoniae using a nanostructured mesoporous silica (SBA-15) as an adjuvant, and compare its effect with an intramuscular (IM) commercial vaccine (CV). Fifty 24 day-old M. hyopneumoniae-free piglets composed five equal groups for different immunization protocols, consisting of a CV and/or oral immunization (OI). Control piglets did not receive any form of immunization. All piglets were challenged with M. hyopneumoniae strain 232 on D49 by tracheal route. IgA antibody response in the respiratory tract, bacterial shedding and serum IgG were evaluated. The piglets were euthanized on 28 (D77) and 56 (D105) days post-infection. Lung lesions were macroscopically evaluated; lung fragments and bronchoalveolar fluid (BALF) were collected for estimation of bacterial loads by qPCR and/or histopathology examination. All immunization protocols induced reduction on Mycoplasma-like macroscopic lung lesions. IgA Ab responses anti-M. hyopneumoniae, the expression of IL-4 cytokine and a lower expression of IL-8 were induced by CV and OI vaccines, while IgG was induced only by CV. Oral immunization using silica as a carrier-adjuvant can be viable in controlling M. hyopneumoniae infection. School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp) Department of Chemistry Federal University of São Paulo (UNIFESP) Butantan Institute Physics Institute University of São Paulo (USP) School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp) FAPESP: #2018/12742-5 FAPESP: #2019/19710-4 |
| Databáze: | OpenAIRE |
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