Influence of Polyamines on Induction of Interferon and Resistance to Viruses by Synthetic Polynucleotides
| Autor: | Maurice R. Hilleman, Lampson Gp, Alfred A. Tytell, Nemes Mm, Field Ak |
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| Rok vydání: | 1969 |
| Předmět: |
Hot Temperature
Time Factors Pneumonia Viral Polynucleotides Spermine Vaccinia virus Kidney Antiviral Agents Vesicular stomatitis Indiana virus General Biochemistry Genetics and Molecular Biology Mice chemistry.chemical_compound Ribonucleases Interferon Culture Techniques Viral Interference medicine Animals Amines Neamine biology Neomycin Haplorhini biology.organism_classification Parainfluenza Virus 1 Human Biochemistry chemistry Virus Diseases Vesicular stomatitis virus Polynucleotide biology.protein Biological Assay Pancreatic ribonuclease Interferons Rabbits Polyamine medicine.drug |
| Zdroj: | Experimental Biology and Medicine. 132:212-218 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | SummaryCertain basic polyamines, such as neomycin, neamine, kanamycin, streptomycin, and spermine, added to poly I:C increased the Tm. The degree of termal stabilization was influenced by the molecular ratio of polyamine to poly I:C and by the ionic strength. Complexing with certain of the polyamines markedly reduced the rate of degradation of poly I:C but not of poly C by pancreatic ribonuclease. Some of the polyamines potentiated induction of resistance by poly I:C of primary rabbit kidney cells but not RK-13 rabbit kidney line or primary grivet kidney cells to infection by vesicular stomatitis virus. Neomycin did not alter the activity of poly I:C in intact animals against pneumonia virus of mice or against parainfluenza 1 (Sendai) virus in mice and did not decrease the toxicity of the complexed polynucleotide for these animals. Added neomycin did not affect the induction by poly I:C of interferon in rabbits. Neomycin did not render the single-stranded RNA's, poly I and poly C, capable of inducing inte... |
| Databáze: | OpenAIRE |
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