Dynamics of formation and morphological features of neutrophil extracellular traps formed under the influence of opsonized Staphylococcus aureus
Autor: | Svetlana N. Pleskova, Ekaterina N. Gorshkova, Ruslan N. Kriukov |
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Rok vydání: | 2018 |
Předmět: |
Adult
Male 0301 basic medicine Staphylococcus aureus Time Factors Neutrophils Primary Cell Culture Microscopy Atomic Force Extracellular Traps Time-Lapse Imaging Histones Focal adhesion 03 medical and health sciences chemistry.chemical_compound Structural Biology Humans Molecular Biology Opsonin chemistry.chemical_classification biology Immune Sera Dynamics (mechanics) Substrate (chemistry) DNA Neutrophil extracellular traps Opsonin Proteins Kinetics 030104 developmental biology Enzyme Histone chemistry biology.protein Biophysics Nucleic Acid Conformation Female |
Zdroj: | Journal of Molecular Recognition. 31:e2707 |
ISSN: | 0952-3499 |
Popis: | In the process of performing their protective functions, neutrophils can form neutrophil extracellular traps (NETs), consisting of DNA in combination with enzymes and histones. The aim of the study was to determine the dynamics of the formation of NETs under the influence of opsonized Staphylococcus aureus and to determine the morphological features of their development in real time by atomic force microscopy. It was found that the maximum formation of NETs was observed after 3 hours of co-incubation of neutrophils and opsonized S. aureus. For the first time, the atomic force microscopy method revealed that, at first, large blocks of parallel DNA helices are formed, which then spread in waves, and only then their bifurcation and separation can be observed. Some of the strands formed are covered by a shell, which subsequently completely disappears. Enzymes and histones become clearly visible only after 140 to 150 minutes of observation. The DNA helixes move toward the opsonized S. aureus. After NET formation, the cell remains on the substrate only in the form of traces of focal adhesion. This, and the fact that the maximum amount of NETs is formed after 3 hours of co-incubation with opsonized S. aureus, suggests that the formation of NETs follows the classical mechanism. The study of the dynamics of formation and the microstructure of NETs makes it possible to estimate the time frame for the implementation of this protective mechanism of the human body when performing the compensatory inflammatory reaction. |
Databáze: | OpenAIRE |
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