An all-in-one magnetic SERS nanosensor for ratiometric detection of Escherichia coli in foods
| Autor: | Lan Jiang, Xiao-Di Hu, Qin-Ling Shi, Yi-Wei Weng, Xiao-Lan Wei |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Silver
Aptamer Metal Nanoparticles Nanoparticle Spectrum Analysis Raman medicine.disease_cause Benzoates Ferric Compounds Biochemistry Analytical Chemistry symbols.namesake Limit of Detection Nanosensor Escherichia coli medicine Animals Humans Sulfhydryl Compounds Escherichia coli Infections Detection limit Chromatography Chemistry Substrate (chemistry) Milk Manganese Compounds Food Microbiology symbols Raman spectroscopy Food Analysis Raman scattering |
| Zdroj: | Analytical and Bioanalytical Chemistry. 413:5419-5426 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-021-03521-2 |
| Popis: | An all-in-one nanosensor was developed for the magnetic enrichment and ratiometric surface-enhanced Raman scattering (SERS) detection of Escherichia coli (E. coli). The all-in-one nanosensor was constructed through the chemical integration of four components into a single nanoparticle, which include a manganese ferrite nanoparticle serving as the magnetic core, a thin silver shell as the SERS substrate, a self-assembled layer of 4-mercaptobenzoic acid (MBA) molecules as the SERS internal standard, and a MBA-conjugated layer of aptamer sequences as the capture probe of E. coli. In the detection of E. coli in food, the target cells were first captured by the nanosensors and magnetically enriched in a short time of 15 min, and then the ratiometric SERS was performed through the Raman intensity ratio between two specific SERS peaks produced by the captured E. coli and the internal MBA. The pre-concentration and ratiometry enabled the nanosensor to detect E. coli with a detection limit down to 10 CFU/mL. The all-in-one nanosensor was successfully applied for the detection of E. coli in various liquid foods including milk, juice, tea, and coffee, with recoveries ranging from 89 to 110% and relative standard deviation lower than 1.7%. In comparison with the previous sandwich strategy adopted by most SERS sensors, this nanosensor endowed with an easier use and a lower cost is more sensitive and reproducible, leading to a great potential in practical applications. |
| Databáze: | OpenAIRE |
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