Tie2 Expressing Monocytes in the Spleen of Patients with Primary Myelofibrosis
| Autor: | Campanelli, R., Fois, G., Catarsi, P., Poletto, V., Villani, L., Erba, B. G., Maddaluno, L., Jemos, B., Salmoiraghi, S., Guglielmelli, P., Abbonante, V., Di Buduo, C. A., Balduini, A., Iurlo, A., Barosi, G., Rosti, V., Massa, M., Vannucchi, A. M., Balliu, M., Bartalucci, N., Bogani, C., Bosi, A., Calabresi, L., Corbizzi Fattori, G., Fanelli, T., Fjerza, R., Gesullo, F., Mannarelli, C., Merli, L., Pacilli, A., Pancrazzi, A., Paoli, C., Pieri, L., Rotunno, G., Sant'Antonio, E., Bonetti, E., Cazzola, M., Ambaglio, I., Bernasconi, P., Casetti, C. I., Catricala, S., Elena, C., Fugazza, E., Galli, A., Malcovati, L., Milanesi, C., Pascutto, C., Pietra, D., Ripamonti, F., Rossi, M., Rumi, E., Dejana, E., Breviario, F., Corada, M., Malinverno, M., Rambaldi, A., Chioda, G., Ferrari, M. L., Finazzi, G., Finazzi, M. C., Belotti, C., Boroni, C., Amaru, A., Golay, J., Bortoluzzi, S., Bisognin, A., Coppe, A., Saccoman, C., Manfredini, R., Artuso, L., Bernardis, I., Bianchi, E., Montanari, M., Pennucci, V., Prudente, Z., Rontauroli, S., Rossi, C., Ruberti, S., Salati, S., Tagliafico, E., Tenedini, E., Zini, R. |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology Physiology Angiogenesis CD34 Gene Expression lcsh:Medicine Medicine (all) Biochemistry Genetics and Molecular Biology (all) Agricultural and Biological Sciences (all) Cardiovascular Physiology Monocytes White Blood Cells 0302 clinical medicine Animal Cells Immune Physiology Medicine and Health Sciences Blood and Lymphatic System Procedures Electron Microscopy lcsh:Science Microscopy Multidisciplinary Neovascularization Pathologic Cell Differentiation Hematology Middle Aged Receptor TIE-2 Haematopoiesis medicine.anatomical_structure 030220 oncology & carcinogenesis Splenectomy cardiovascular system Female Cellular Types Receptor Research Article medicine.medical_specialty Aged Case-Control Studies Humans Primary Myelofibrosis Spleen Patients Immune Cells CD14 Immunology Surgical and Invasive Medical Procedures Biology Research and Analysis Methods 03 medical and health sciences Genetics medicine Progenitor cell TIE-2 Myelofibrosis Neovascularization Pathologic Blood Cells lcsh:R Biology and Life Sciences Cell Biology medicine.disease Hematopoiesis Health Care 030104 developmental biology Transmission Electron Microscopy lcsh:Q Bone marrow Developmental Biology |
| Zdroj: | PLoS ONE, Vol 11, Iss 6, p e0156990 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0156990 |
| Popis: | Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34+ progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14+ cells are Tie2+ and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2+CD14lowCD16brightCDL62-CCR2- (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14+ cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14+Tie2+ cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2+ monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ. |
| Databáze: | OpenAIRE |
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