Increased neuronal nitric oxide synthase expression in brain following portacaval anastomosis

Autor:	R. M. Audet, Roger F. Butterworth, V. L. Raghavendra Rao
Rok vydání:	1997
Předmět:	Male medicine.medical_specialty Blotting Western Molecular Sequence Data Portacaval Portacaval shunt Biology Nitroarginine Polymerase Chain Reaction Gene Expression Regulation Enzymologic Nitric oxide Rats Sprague-Dawley Pathogenesis chemistry.chemical_compound Western blot Internal medicine medicine Animals Enzyme Inhibitors Molecular Biology reproductive and urinary physiology DNA Primers Neurons medicine.diagnostic_test Portacaval Shunt Surgical General Neuroscience Portacaval anastomosis Brain musculoskeletal system Rats body regions Nitric oxide synthase Endocrinology nervous system Cerebral blood flow Biochemistry chemistry cardiovascular system biology.protein RNA Neurology (clinical) Nitric Oxide Synthase Developmental Biology
Zdroj:	Brain Research. 765:169-172
ISSN:	0006-8993
DOI:	10.1016/s0006-8993(97)00652-5
Popis:	It has previously been suggested that increases of l-arginine uptake into brain following portacaval shunting may result in increased activities of constitutive neuronal nitric oxide synthase (nNOS). In order to further address this issue, nNOS protein and gene expression were studied by Western blot analysis using a monoclonal nNOS antibody and RT-PCR respectively in the brains of rats following portacaval shunting or sham operation. Portacaval shunting resulted in a 2-fold increase (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed4c800099cb0b0a89c37bf6de2ae024 https://doi.org/10.1016/s0006-8993(97)00652-5 Zobrazit plný text záznamu Full Text from ScienceDirect