Evaluation of Cationic Liposomes for Delivery of Diphtheria Toxin A-Chain Gene to Cells Infected with Bovine Leukemia Virus
| Autor: | Dan-Dan Zhao, Tana, Misao Onuma, Hitoshi Kakidani, Shinobu Watarai, Jin-tae Lee, Tatsuji Yasuda |
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| Rok vydání: | 1997 |
| Předmět: |
Genes
Viral viruses Gene delivery Kidney Transfection Cations Leukemia Virus Bovine Animals Deoxyribonuclease I Diphtheria Toxin Luciferase Cationic liposome Luciferases Cell Line Transformed Electrophoresis Agar Gel Diphtheria toxin Drug Carriers Liposome Sheep General Veterinary Bovine leukemia virus biology Cationic polymerization Genetic Therapy Enzootic Bovine Leukosis biology.organism_classification Molecular biology Peptide Fragments Liposomes Cattle Plasmids |
| Zdroj: | Journal of Veterinary Medical Science. 59:169-174 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.59.169 |
| Popis: | We investigated whether cationic liposomes are efficient at delivering the gene for diphtheria toxin A-chain (DT-A) under the control of the long terminal repeat (LTR) of bovine leukemia virus (BLV) (pLTR-DT) into BLV-infected cells and are also suitable for in vivo use. The transfection activity of the cationic liposomes composed of N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dioleoyl phosphatidylethanolamine (DOPE) and dilauroyl phosphatidylcholine (DLPC) (1:2:2, molar ratio) (TMAG-liposome) and liposomes composed of phosphatidylserine (PS) (PS-liposome) was evaluated by the luciferase assay using a plasmid which contains the coding sequence of firefly luciferase under the control of the SR alpha promoter (pSR alpha/L-A delta 5). The TMAG-liposome gave highly efficient transfection in the presence of serum. On the other hand, PS-liposome showed inferior efficiency. When BLV-infected cells were co-transfected with a fixed amount of pSR alpha/L-A delta 5-entrapped TMAG-liposome and various amount of pLTR-DT-containing TMAG-liposome, the luciferase activity in the BLV-infected cells was inhibited by the addition of pLTR-DT-entrapped TMAG-liposome dose-dependently. The cationic TMAG-liposome containing pLTR-DT was successively added to BLV-infected cells in culture. The number of viable cells was markedly reduced by the cationic TMAG-liposome containing pLTR-DT. On the other hand, TMAG-liposome containing pSR alpha/L-A delta 5 showed no such effect. pLTR-DT entrapped by the cationic TMAG-liposome was not digested by the treatment with DNase I and with serum. These results suggest that the cationic liposomes, such as TMAG-liposome, may be efficient transfection reagent for the BLV-infected cells and can be utilized for DT-A gene delivery into the BLV-infected cells in vivo. |
| Databáze: | OpenAIRE |
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