Thrombin induces production of growth factors from aortic smooth muscle cells
| Autor: | Valentina Corvino, Anna Di Carlo, Sabrina Pagliei, Alessandra Cucina, Antonio V. Sterpetti, Valeria Borrelli, Antonino Cavallaro, Luciana Santoro-D'Angelo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Platelet-derived growth factor
medicine.medical_treatment Thoracic Basic fibroblast growth factor Aorta Thoracic Muscle Smooth Vascular chemistry.chemical_compound Conditioned Monoclonal Myocyte Settore BIO/13 - BIOLOGIA APPLICATA Aorta Cells Cultured Platelet-Derived Growth Factor Cultured biology Antibodies Monoclonal Arteries Hyperplasia PDGF thrombin Muscle Fibroblast Growth Factor 2 Smooth Platelet-derived growth factor receptor Cell Division medicine.drug medicine.medical_specialty bFGF Growth factors Smooth muscle cell Thrombin Animals Cattle Culture Media Conditioned DNA Humans Kinetics Cells Antibodies Internal medicine Vascular growth factors medicine smooth muscle cell Growth factor medicine.disease Culture Media Endocrinology chemistry Cell culture biology.protein Surgery |
| Popis: | Myointimal hyperplasia is a common complication of arterial recontructive surgery. The serine protease thrombin has a major role in vessel wall healing and eventual myointimal hyperplasia formation. The aim of this study was to determine the effect of thrombin on the production of PDGF AA and bFGF by arterial smooth muscle cells.Bovine smooth muscle cells were stimulated with thrombin in a serum-free culture. The release of PDGF AA and bFGF was assessed by ELISA. The effect of thrombin on the proliferation of confluent monolayers of bovine smooth muscle cells was determined by tritiated thymidine uptake.Smooth muscle cells stimulated with thrombin released more PDGF AA (P0.001) and bFGF (P0.001) than the control. Addition of anti-PDGF AA and anti-bFGF antibodies to the medium of smooth muscle cell cultures neutralized the mitogenic effect of thrombin (P0.001).The findings of our study suggest that thrombin may lead to myointimal hyperplasia formation through induction of PDGF and bFGF production by smooth muscle cells. |
| Databáze: | OpenAIRE |
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