Quantitative Proteomics Reveals Myosin and Actin as Promising Saliva Biomarkers for Distinguishing Pre-Malignant and Malignant Oral Lesions
| Autor: | Nelson L. Rhodus, Frank G. Ondrey, Joel A. Kooren, Robert J. Griffin, Hongwei Xie, Baolin Wu, Timothy J. Griffin, Matthew D. Stone, Eric W. Refsland, Chap T. Le, Getiria Onsongo, Xiaobing Chen, Ebbing P. de Jong, John V. Carlis |
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| Rok vydání: | 2010 |
| Předmět: |
Proteomics
Saliva Pathology medicine.medical_specialty Quantitative proteomics lcsh:Medicine Myosins Biology Malignancy Sensitivity and Specificity 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Myosin medicine Humans lcsh:Science Chromatography High Pressure Liquid Actin 030304 developmental biology 0303 health sciences Chemistry/Physical Inorganic and Analytical Chemistry Oncology/Head and Neck Cancers Multidisciplinary lcsh:R Cancer medicine.disease Molecular biology Actins 3. Good health Blot 030220 oncology & carcinogenesis lcsh:Q Biotechnology/Protein Chemistry and Proteomics Mouth Neoplasms Precancerous Conditions Biomarkers Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 6, p e11148 (2010) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0011148 |
| Popis: | Background Oral cancer survival rates increase significantly when it is detected and treated early. Unfortunately, clinicians now lack tests which easily and reliably distinguish pre-malignant oral lesions from those already transitioned to malignancy. A test for proteins, ones found in non-invasively-collected whole saliva and whose abundances distinguish these lesion types, would meet this critical need. Methodology/Principal Findings To discover such proteins, in a first-of-its-kind study we used advanced mass spectrometry-based quantitative proteomics analysis of the pooled soluble fraction of whole saliva from four subjects with pre-malignant lesions and four with malignant lesions. We prioritized candidate biomarkers via bioinformatics and validated selected proteins by western blotting. Bioinformatic analysis of differentially abundant proteins and initial western blotting revealed increased abundance of myosin and actin in patients with malignant lesions. We validated those results by additional western blotting of individual whole saliva samples from twelve other subjects with pre-malignant oral lesions and twelve with malignant oral lesions. Sensitivity/specificity values for distinguishing between different lesion types were 100%/75% (p = 0.002) for actin, and 67%/83% (p |
| Databáze: | OpenAIRE |
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