A method for topical dosing of invertebrates with pesticide for use in feeding experiments
| Autor: | Andrew C. Warden, Kimberly Maute, Grant C. Hose, Greg Dojchinov, Kristine French, Paul Story |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Insecticides Serial dilution Health Toxicology and Mutagenesis 010501 environmental sciences Management Monitoring Policy and Law Biology Toxicology 01 natural sciences Predation Fenitrothion chemistry.chemical_compound Ecotoxicology Animals Pesticides Fipronil 0105 earth and related environmental sciences Residue (complex analysis) Australia General Medicine Pesticide biology.organism_classification Invertebrates 010602 entomology chemistry Acheta |
| Zdroj: | Ecotoxicology (London, England). 30(2) |
| ISSN: | 1573-3017 |
| Popis: | The ability to produce large numbers of pesticide-exposed insects (e.g. crickets) is important for feeding studies into the effects of pesticides on key predatory species. House crickets (Acheta domesticus L. 1758) were submersed in serial dilutions of the pesticides, fenitrothion and fipronil, used for the control of locusts in Australia, and then rapidly frozen for residue analysis. Good correlations were found between increasing concentrations of serial pesticide dilutions and the resultant residual concentrations of the parent compounds in crickets, with R2 values of 0.949 (fenitrothion) and 0.946 (fipronil). R2 values for the much less abundant fipronil metabolites were lower 0.858 (sulfone), 0.368 (desulfinyl) and 0.785 (sulfide). This method enables insecticide exposure mimicking the field conditions to be assessed, and can be done immediately prior to an experiment. This ensures locusts remain alive when introduced to the feeding chambers, and enables multiple prey items to be dosed with a known pesticide burden. |
| Databáze: | OpenAIRE |
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