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Background plant polyphenols have poor water solubility, resulting in low bioavailability. In order to overcome this limitation, the drug molecules can be coated with multiple layers of polymeric materials. Methods microcrystals of quercetin and resveratrol coated with a (PAH/PSS)4 or (CH/DexS)4 shell were prepared using the layer-by-layer assembly method; cultured human HaCaT keratinocytes were treated with UV-C and after that cells were incubated with native and particulate polyphenols. DNA damage, cell viability and integrity, were evaluated by comet-assay, using PrestoBlueTM reagent and lactate dehydrogenase (LDH) leakage test. Results the data obtained indicate that: both native and particulate polyphenols added immediately after UV-C exposure increased cell viability in a dose-dependent manner, however the efficiency of particulate polyphenols was more pronounced; quercetin coated with a (PAH/PSS)4 or (CH/DexS)4 shell more effectively than the native compound reduced the number of single-strand DNA breaks in the nuclei of keratinocytes exposed to UV-C radiation; native and particulate resveratrol were ineffective against DNA damage. Conclusion quercetin reduces cell death caused by UV-C radiation and increase DNA repair capacity. Coating quercetin with (PAH/PSS)4 or (CH/DexS)4 markedly enhanced its impact on DNA repair probably by facilitating transport into the nucleus. |
