pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
| Autor: | Marcus Persicke, Susanne Schneiker-Bekel, Alfred Pühler, Jörn Kalinowski, Lucas Jacob, Camilla März, Lena Schaffert, Christian Rückert |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Actinoplanes sp. SE50/110
0303 health sciences biology 030306 microbiology Computational biology biology.organism_classification Actinoplanes sp Bacteriophage 03 medical and health sciences Immunology and Microbiology (miscellaneous) Transcription (biology) Genetics Vector system Actinomycetales Expression cassette Molecular Biology Gene 030304 developmental biology |
| Popis: | The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter. |
| Databáze: | OpenAIRE |
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