Visible and near infrared resonance plasmonic enhanced nanosecond laser optoporation of cancer cells
| Autor: | Étienne Boulais, Bastien St-Louis Lalonde, Michel Meunier, Jean-Jacques Lebrun |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Materials science
Membrane permeability business.industry Scanning electron microscope ocis:(140.3538) Lasers pulsed Perforation (oil well) Laser Fluorescence Atomic and Molecular Physics and Optics law.invention Cell Studies chemistry.chemical_compound Optics chemistry Colloidal gold law Optoelectronics Propidium iodide Irradiation business Biotechnology ocis:(170.0170) Medical optics and biotechnology |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | In this paper, we report a light driven, non-invasive cell membrane perforation technique based on the localized field amplification by a nanosecond pulsed laser near gold nanoparticles (AuNPs). The optoporation phenomena is investigated with pulses generated by a Nd:YAG laser for two wavelengths that are either in the visible (532 nm) or near infrared (NIR) (1064 nm). Here, the main objective is to compare on and off localized surface plasmonic resonance (LSPR) to introduce foreign material through the cell membrane using nanosecond laser pulses. The membrane permeability of human melanoma cells (MW278) has been successfully increased as shown by the intake of a fluorescent dye upon irradiation. The viability of this laser driven perforation method is evaluated by propidium iodide exclusion as well as MTT assay. Our results show that up to 25% of the cells are perforated with 532 nm pulses at 50 mJ/cm(2) and around 30% of the cells are perforated with 1064 nm pulses at 1 J/cm(2). With 532 nm pulses, the viability 2 h after treatment is 64% but it increases to 88% 72 h later. On the other hand, the irradiation with 1064 nm pulses leads to an improved 2 h viability of 81% and reaches 98% after 72 h. Scanning electron microscopy images show that the 5 pulses delivered during treatment induce changes in the AuNPs size distribution when irradiated by a 532 nm beam, while this distribution is barely affected when 1064 nm is used. |
| Databáze: | OpenAIRE |
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