Examining transmission of gut bacteria to preserved carcass via anal secretions in Nicrophorus defodiens
| Autor: | Lindsay M. Gielda, Christopher James Miller, J. Curtis Creighton, Scott T. Bates |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Bodily Secretions Physiology Nicrophorus defodiens Anal Canal Pathology and Laboratory Medicine 01 natural sciences Beetles Medicine and Health Sciences DNA extraction Larva Multidisciplinary biology Transmission (medicine) digestive oral and skin physiology Vertebrate food and beverages Eukaryota High-Throughput Nucleotide Sequencing Genomics Biodiversity Bacterial Pathogens Insects Coleoptera Medical Microbiology Burying beetle Medicine Anatomy Pathogens Research Article Arthropoda Science Zoology Microbial Genomics 010603 evolutionary biology Microbiology 03 medical and health sciences Extraction techniques biology.animal Genetics Animals Microbiome Microbial Pathogens Illumina dye sequencing Secretion Clostridium Bacteria Gut Bacteria Organisms Biology and Life Sciences biology.organism_classification Invertebrates Gastrointestinal Microbiome Gastrointestinal Tract Research and analysis methods 030104 developmental biology Physiological Processes Digestive System |
| Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 12, p e0225711 (2019) |
| ISSN: | 1932-6203 |
| Popis: | Direct transmission of bacteria to subsequent generations highlights the beneficial nature of host-bacteria relationships. In insects, this process is often mediated by the production of microbe-containing secretions. The objective of this study was to determine if the burying beetle, Nicrophorus defodiens, utilizes anal secretions to transmit adult digestive tract bacteria onto a small vertebrate carcass; thus creating the potential to aid in carcass preservation or pass digestive tract bacteria to their larval offspring. Using high-throughput Illumina sequencing of the 16S rRNA gene, we characterized bacterial communities of adult beetle digestive tracts, their anal secretions, and prepared mouse carcasses. We also examined unprepared carcass bacterial communities as a means to interpret community shifts that take place during carcass preservation. We found a vast reduction in diversity on prepared carcasses after anal secretion application. Overall, there was little similarity in bacterial communities among adult digestive tracts, anal secretions, and prepared carcasses, suggesting bacterial communities found in adult digestive tracts do not successfully colonize and achieve dominance on prepared carcasses by way of beetle anal secretions. We concluded that N. defodiens does not transmit their digestive tract bacterial communities to prepared carcasses in a wholesale manner, but may transmit key microbes, including core microbiome members, to preserved carcasses that may ultimately act to sustain larvae and serve as inocula for larval digestive tracts. |
| Databáze: | OpenAIRE |
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