Flow cytometry for age assessment of a yeast population and its application in beer fermentations
| Autor: | Martin Baszczyňski, Radek Lehnert, Tomáš Brányik, Michal Kuřec, José A. Teixeira, André Mota |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Předmět: |
Aging
Population Industrial fermentation Biology Flow cytometry Microbiology 03 medical and health sciences medicine Food science education 030304 developmental biology Alexa Fluor Staining 0303 health sciences education.field_of_study Science & Technology medicine.diagnostic_test 030306 microbiology business.industry food and beverages Beer Bud scar Yeast Brewing Fermentation business Food Science |
| Zdroj: | Scopus-Elsevier Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 6046-1373 |
| Popis: | An expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed a good correlation and allowed routine cell age estimation by flow cytometry. The developed method was applied for yeast age control in traditional batch and continuous beer fermentations. At the pitching rates used in industrial beer fermentations, our results support former findings by locating a gradient of increasing yeast age from the top to the bottom zone of the fermenter cone. The results also indicate that in continuous beer fermentation, the increasing bud scar fluorescence of immobilized cells could help to schedule the replacement of aged biomass, prior to loss of viability or deterioration of process performance and product quality. Grant Agency of the Czech Republic (GAČR 104/06/1418) and MŠMT (MSM 6046137305, Czech Republic). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|