Increase in primary cilia in the epidermis of patients with atopic dermatitis and psoriasis
| Autor: | Fumitaka Fujita, Akimichi Morita, Okada Fumihiro, Ken Ishii, Runa Fukui, Defri Rizaldy, Toriyama Manami, Motoki Nakamura, Hiroko Kato |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Keratinocytes
0301 basic medicine p38 mitogen-activated protein kinases keratinocyte Inflammation Dermatology Biochemistry Dermatitis Atopic 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Psoriasis Ciliogenesis Humans Medicine Cilia Molecular Biology Cells Cultured Epidermis (botany) business.industry Cilium epidermal cilia Atopic dermatitis medicine.disease 030104 developmental biology medicine.anatomical_structure inflammation Immunology Cytokines JNK Epidermis medicine.symptom business Keratinocyte IFT88 |
| Zdroj: | Experimental Dermatology. 30:792-803 |
| ISSN: | 1600-0625 0906-6705 |
| Popis: | Background Primary cilia influence cell activity, and thus have a unique role in maintaining cell proliferation and differentiation. In atopic dermatitis (AD) and psoriasis, areas of skin inflammation exhibit dysregulated keratinocyte homeostasis. The role of primary cilia in these conditions remains unclear. Objective To elucidate the incidence of primary cilia in skin inflammation and the potential mechanism underlying the dysregulation of keratinocytes. Methods Primary cilia were observed using immunofluorescence staining. Normal skin samples were compared with skin samples from patients with AD or psoriasis in terms of cilia numbers and length. The effect of cytokine stimulation on ciliogenesis in keratinocytes was analyzed using a primary keratinocyte culture. IFT88, an important ciliary intraflagellar protein, was blocked in Th2 and Th17 cytokines-stimulated keratinocytes. These effects were analyzed with quantitative polymerase chain reaction and Western blot. Results Significant increases in ciliated cells were observed in AD and psoriasis skin samples compared to normal skin samples. The stimulation of keratinocytes using Th2 and Th17 cytokines modulated the formation of primary cilia. The amount of IFT88 in the primary cilia associated with the phosphorylation of JNK, but not p38, in keratinocytes stimulated with interleukin-13, 17A, and 22. Conclusion An increase of ciliated cells in the epidermis may impair keratinocyte differentiation under stress conditions caused by inflammation in both AD and psoriasis patients. |
| Databáze: | OpenAIRE |
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