Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart
| Autor: | Iwona Bil-Lula, Anna Krzywonos-Zawadzka, Magdalena Wawrzyńska, Mieczyslaw Wozniak, Grzegorz Sawicki, Dariusz Biały, Virgilio J. J. Cadete |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Heart Injury animal structures Article Subject Ischemia lcsh:Medicine Myocardial Reperfusion Injury General Biochemistry Genetics and Molecular Biology Contractility Coronary circulation Electromagnetic Fields Organ Culture Techniques Coronary Circulation Internal medicine Troponin I Heart rate medicine Animals Humans chemistry.chemical_classification Reactive oxygen species General Immunology and Microbiology Interleukin-6 Myocardium lcsh:R General Medicine medicine.disease Myocardial Contraction Rats medicine.anatomical_structure Rate pressure product chemistry Cardiology Matrix Metalloproteinase 2 Reactive Oxygen Species Research Article |
| Zdroj: | BioMed Research International, Vol 2015 (2015) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2015/396593 |
| Popis: | Low frequency electromagnetic field (LF-EMF) decreases the formation of reactive oxygen species, which are key mediators of ischemia/reperfusion (I/R) injury. Therefore, we hypothesized that the LF-EMF protects contractility of hearts subjected to I/R injury. Isolated rat hearts were subjected to 20 min of global no-flow ischemia, followed by 30 min reperfusion, in the presence or absence of LF-EMF. Coronary flow, heart rate, left ventricular developed pressure (LVDP), and rate pressure product (RPP) were determined for evaluation of heart mechanical function. The activity of cardiac matrix metalloproteinase-2 (MMP-2) and the contents of coronary effluent troponin I (TnI) and interleukin-6 (IL-6) were measured as markers of heart injury. LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow. In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF. Although TnI and IL-6 levels were increased in I/R hearts, their levels returned to baseline aerobic levels in I/R hearts subjected to LF-EMF. The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF. The data presented here indicate that acute exposure to LF-EMF protects mechanical function of I/R hearts and reduces I/R injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|