Particulate matter exposure is associated with inflammatory gene methylation in obese subjects

Autor: Ilaria Restelli, Letizia Tarantini, Valentina Bollati, Luisella Vigna, Simona Iodice, Benedetta Albetti, Laura Cantone, Matteo Bonzini, Angela Cecilia Pesatori
Rok vydání: 2017
Předmět:
Adult
Male
0301 basic medicine
Inflammation
BMI
Body mass index
010501 environmental sciences
Biology
Overweight
PM
particulate matter
01 natural sciences
Biochemistry
Article
TLR4
toll-like receptor 4
Pathogenesis
03 medical and health sciences
Environmental Science(all)
medicine
Humans
Obesity
Particle Size
Adverse effect
Aged
0105 earth and related environmental sciences
General Environmental Science
TNF
tumor necrosis factor
DNA methylation
PM10
particulate matter with aerodynamic diameters <10 µm
PM exposure
Pyrosequencing
Middle Aged
medicine.disease
3. Good health
NF-кB
nuclear factor κB
030104 developmental biology
Italy
Immunology
TLR4
LPS
lipopolysaccharide
Environmental Pollutants
Female
Particulate Matter
Tumor necrosis factor alpha
medicine.symptom
CD14
cluster of differentiation 14
Blood Chemical Analysis
Zdroj: Environmental Research
ISSN: 0013-9351
DOI: 10.1016/j.envres.2016.11.002
Popis: Background Overweight and obesity are becoming more widespread with alarming projections for the coming years. Obesity may increase susceptibility to the adverse effects of PM exposure, exacerbating the effects on cardiovascular diseases and altering the biomarkers of vascular inflammation. The associated biological mechanisms have not been fully understood yet; the common denominator in the pathogenesis of the co-morbidities of obesity is the presence of an active, low-grade inflammatory process. DNA methylation has been shown to regulate inflammatory pathways that are responsible for the development of cardiovascular diseases. Objectives The aim of the study was to investigate, in a population of overweight/obese subjects, the effects of PM on blood DNA methylation in genes associated to inflammatory response. Methods Using bisulfite pyrosequencing, we measured DNA methylation in peripheral blood mononuclear cells from 186 overweighted/obese subjects. In particular, we quantified DNA methylation in a set of 3 candidate genes, including CD14, TLR4 and TNF-α, because of the important roles that these genes play in the inflammatory pathway. Personal exposure to PM10 was estimated for each subject based on the local PM10 concentrations, measured by monitoring stations at residential address. Repeated measure models were used to evaluate the association of PM10 with each genes, accounting for possible correlations among the genes that regulate the same inflammatory pathway. Results We found an inverse association between the daily PM10 exposure and the DNA methylation of inflammatory genes, measured in peripheral blood of healthy overweight/obese subjects. Considering different exposure time-windows, the effect on CD14 and TLR4 methylation was observed, respectively, in days 4–5-6, and days 6–7-8. TNF-α methylation was not associated to PM10. Conclusions Our findings support a picture in which PM10 exposure and transcriptional regulation of inflammatory gene pathway in obese subjects are associated.
Highlights • Overweight/obese subjects has been proposed as susceptible population for PM related effects. • DNA methylation is a key molecular mechanisms linking PM exposure to systemic pro-inflammatory effects • PM10 exposure resulted associated to DNA methylation of inflammatory genes in a population of obese patients. • The relationship between PM10 and DNA methylation of inflammation pathway-genes was confirmed in obese subjects.
Databáze: OpenAIRE
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