Stimulated phospholipid synthesis is key for hepatitis B virus replications
| Autor: | Hehua Lei, Laifeng Ding, Yulan Wang, Qingxia Huang |
|---|---|
| Přispěvatelé: | Lee Kong Chian School of Medicine (LKCMedicine), Singapore Phenome Center |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male HBsAg Hepatitis B virus Phospholipid Phosphatidate Phosphatase lcsh:Medicine medicine.disease_cause Virus Replication Virus Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Metabolomics Humans Medicine [Science] Phosphatidylinositol Choline-Phosphate Cytidylyltransferase Hepatitis B e Antigens lcsh:Science Phosphatidylethanolamine Multidisciplinary Lipogenesis lcsh:R virus diseases Phosphatidylserine Hep G2 Cells Middle Aged Hepatitis B Virology digestive system diseases Hepatitis B Virus (HBV) 030104 developmental biology chemistry Viral replication Viral infection 030220 oncology & carcinogenesis lcsh:Q Female |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Chronic hepatitis B Virus (HBV) infection has high morbidity, high pathogenicity and unclear pathogenesis. To elucidate the relationship between HBV replication and host phospholipid metabolites, we measured 10 classes of phospholipids in serum of HBV infected patients and cells using ultra performance liquid chromatograph-triple quadruple mass spectrometry. We found that the levels of phosphatidylcholine (PC), phosphatidylethanolamine, and lyso-phosphatidic acid were increased in HBsAg (+) serum of infected patients compared with HBsAg (−), while phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and sphingomyelin were decreased, which were confirmed in an HBV infected HepG2.2.15 cell line. We further evaluated the enzyme levels of PC pathways and found that PCYT1A and LPP1 for PC synthesis were up-regulated after HBV infection. Moreover, HBV replication was inhibited when PCYT1A and LPP1 were inhibited. These results indicated that the PC synthesis in HBV infected host are regulated by PCYT1A and LPP1, which suggests that PCYT1A, LPP1 could be new potential targets for HBV treatment. |
| Databáze: | OpenAIRE |
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