Microbial community composition and PAHs removal potential of indigenous bacteria in oil contaminated sediment of Taean coast, Korea
| Autor: | Bong-Oh Kwon, Hanbyul Lee, Dong Wan Lee, Jong Seong Khim, Un Hyuk Yim, Jae Jin Kim, Beom Seok Kim, Aslan Hwanhwi Lee |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Geologic Sediments Firmicutes Health Toxicology and Mutagenesis 010501 environmental sciences Toxicology 01 natural sciences Dioxygenases Actinobacteria 03 medical and health sciences Bioremediation Bacterial Proteins Multienzyme Complexes RNA Ribosomal 16S Republic of Korea Petroleum Pollution Polycyclic Aromatic Hydrocarbons 0105 earth and related environmental sciences Bacteria biology Bacteroidetes Biodiversity General Medicine biology.organism_classification Pollution Biodegradation Environmental Petroleum 030104 developmental biology Microbial population biology Metagenomics Environmental chemistry Environmental science Proteobacteria |
| Zdroj: | Environmental Pollution. 234:503-512 |
| ISSN: | 0269-7491 |
| DOI: | 10.1016/j.envpol.2017.11.097 |
| Popis: | The tidal flats near Sinduri beach in Taean, Korea, have been severely contaminated by heavy crude oils due to the Korea's worst oil spill accident, say the Hebei Spirit Oil Spill, in 2007. Crude oil compounds, including polycyclic aromatic hydrocarbons (PAHs), pose significant environmental damages due to their wide distribution, persistence, high toxicity, mutagenicity, and carcinogenicity. Microbial community of Sinduri beach sediments samples was analyzed by metagenomic data with 16S rRNA gene amplicons. Three phyla (Proteobacteria, Firmicutes, and Bacteroidetes) accounted for approximately ≥93.0% of the total phyla based on metagenomic analysis. Proteobacteria was the dominant phylum in Sinduri beach sediments. Cultivable bacteria were isolated from PAH-enriched cultures, and bacterial diversity was investigated through performing culture characterization followed by molecular biology methods. Sixty-seven isolates were obtained, comprising representatives of Actinobacteria, Firmicutes, α- and γ-Proteobacteria, and Bacteroidetes. PAH catabolism genes, such as naphthalene dioxygenase (NDO) and aromatic ring hydroxylating dioxygenase (ARHDO), were used as genetic markers to assess biodegradation of PAHs in the cultivable bacteria. The ability to degrade PAHs was demonstrated by monitoring the removal of PAHs using a gas chromatography mass spectrometer. Overall, various PAH-degrading bacteria were widely present in Sinduri beach sediments and generally reflected the restored microbial community. Among them, Cobetia marina, Rhodococcus soli, and Pseudoalteromonas agarivorans were found to be significant in degradation of PAHs. This large collection of PAH-degrading strains represents a valuable resource for studies investigating mechanisms of PAH degradation and bioremediation in oil contaminated coastal environment, elsewhere. |
| Databáze: | OpenAIRE |
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