The Proto-oncoprotein SYT Interacts with SYT-interacting Protein/Co-activator Activator (SIP/CoAA), a Human Nuclear Receptor Co-activator with Similarity to EWS and TLS/FUS Family of Proteins
| Autor: | Michelle D. Garrett, Catherine J.E. Ingram, Graham H. Goodwin, Per Antonson, Michela Perani, Rifat Hamoudi, Colin Cooper |
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| Rok vydání: | 2005 |
| Předmět: |
Cytoplasm
Transcription Genetic Glutamine Amino Acid Motifs Cell Cycle Proteins RNA-binding protein Ligands Biochemistry Translocation Genetic Protein structure Transcription (biology) Chlorocebus aethiops Cloning Molecular Glutathione Transferase Ribonucleoprotein Serine-Arginine Splicing Factors Intracellular Signaling Peptides and Proteins RNA-Binding Proteins Chromatin Neoplasm Proteins COS Cells RNA splicing Drosophila Plasmids Protein Binding Binding domain Transcriptional Activation DNA Complementary Proline RNA Splicing Recombinant Fusion Proteins Immunoblotting Molecular Sequence Data Glycine Down-Regulation Biology Transfection Models Biological Cell Line Sarcoma Synovial Cell Line Tumor Proto-Oncogene Proteins Two-Hybrid System Techniques Animals Humans Immunoprecipitation Amino Acid Sequence Molecular Biology Gene Library Cell Nucleus Sequence Homology Amino Acid Activator (genetics) Cell Biology Molecular biology Hormones Protein Structure Tertiary Repressor Proteins RNA-Binding Protein FUS Tyrosine RNA-Binding Protein EWS |
| Zdroj: | Journal of Biological Chemistry. 280:42863-42876 |
| ISSN: | 0021-9258 |
| Popis: | The proto-oncoprotein SYT is involved in the unique translocation t(X;18) found in synovial sarcoma SYT-SSX fusions. SYT has a conserved N-terminal domain (SNH domain) that interacts with the human paralog of Drosophila Brahma (hBRM) and Brahma-related gene 1 (BRG1) chromatin remodeling proteins and a C-terminal transactivating sequence rich in glutamine, proline, glycine, and tyrosine (QPGY domain). Here we reported the isolation of the ribonucleoprotein SYT-interacting protein/co-activator activator (SIP/CoAA), which specifically binds the QPGY domain of SYT and also the SYT-SSX2 translocation fusion. SIP/CoAA is a general nuclear co-activator and an RNA splicing modulator that contains two RNA recognition motifs and multiple hexapeptide repeats. We showed that the region consisting of the hexapeptide motif (YQ domain) is similar to the hexapeptide repeat domain found in EWS and in TLS/FUS family proteins. The YQ domain also resembles the QPGY region of SYT itself and like all these other domains acts as a transcriptional activator in reporter assays. Most interestingly, the last 84 amino acids adjacent to YQ down-modulate by 25-fold the YQ transactivation of the reporter gene, and both domains are important for SIP/CoAA binding to SYT. In addition, SYT acts together with SIP/CoAA in stimulating estrogen and glucocorticoid receptor-dependent transcriptional activation. Activation is hormone-dependent and requires functional hBRM and/or BRG1. The stimulation is strongly reduced if the N-terminal region of hBRM/BRG1 (amino acids 1-211) is deleted. This region encompasses the SNF11 binding domain (amino acids 156-211), which interacts specifically with SYT in vivo and in vitro. |
| Databáze: | OpenAIRE |
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