Molecular cloning, sequencing and expression in Escherichia coli of the odontoglossum ringspot virus coat protein gene
| Autor: | Masaaki Chatani, Narinobu Inouye, Masato Ikegami, Akiko Murayama, Yoshinori Isomura, Yoshinori Matumoto |
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| Rok vydání: | 1991 |
| Předmět: |
Gene Expression Regulation
Viral viruses Blotting Western Molecular Sequence Data Biology Plant Viruses Open Reading Frames Capsid Virology Plant virus Escherichia coli Tobacco mosaic virus Amino Acid Sequence Cloning Molecular Peptide sequence Gene Repetitive Sequences Nucleic Acid Base Sequence Odontoglossum ringspot virus Nucleic acid sequence Tobamovirus biology.organism_classification Molecular biology Tobacco Mosaic Virus Open reading frame RNA Viral Electrophoresis Polyacrylamide Gel |
| Zdroj: | Journal of General Virology. 72:2247-2249 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-72-9-2247 |
| Popis: | The sequence of the 3'-terminal 1865 nucleotides of the genome of the tobamovirus odontoglossum ringspot virus (ORSV) was determined. This sequence contained two open reading frames (ORFs), 912 and 477 nucleotides long. The 912 nucleotide ORF has been identified as the cell-to-cell transport protein gene. The 477 nucleotide ORF was expressed in Escherichia coli, and the product was detected by antibodies specific for the coat protein of ORSV. The amino acid sequence of protein encoded by this ORF shares 84% similarity with the tobacco mosaic virus (TMV) (vulgare) coat protein. The 3'-terminal untranslated region of ORSV comprises 414 nucleotides, 210 nucleotides more than that of TMV (vulgare) RNA. |
| Databáze: | OpenAIRE |
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