Performance of a Modified In-House HIV-1 Avidity Assay among a Cohort of Newly Diagnosed HIV-1 Infected Individuals and the Effect of ART on the Maturation of HIV-1 Specific Antibodies
| Autor: | Jaiprasath Sachithanandham, Priscilla Rupali, Punitha Victor, Gnanadurai John Fletcher, Veena Vadhini Ramalingam, John Paul Demosthenes, Tennison Inba Raj Williams, Swaminathan Prasannakumar, Diviya Alex, Rajesh Kannangai |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
030106 microbiology Antibody Affinity Human immunodeficiency virus (HIV) Enzyme-Linked Immunosorbent Assay HIV Infections chemical and pharmacologic phenomena HIV Antibodies medicine.disease_cause Sensitivity and Specificity Cohort Studies 03 medical and health sciences 0302 clinical medicine Immune system Virology Humans Medicine Avidity 030212 general & internal medicine Receiver operating characteristic biology business.industry Specific antibody Infectious Diseases Anti-Retroviral Agents Antibody Formation Immunology Cohort HIV-1 biology.protein Antibody business Viral load |
| Zdroj: | Current HIV Research. 17:134-145 |
| ISSN: | 1570-162X |
| DOI: | 10.2174/1570162x17666190712125606 |
| Popis: | Background:Viral kinetics impact humoral immune response to HIV; antibody avidity testing helps distinguish recent (Method:Commercial LAg Avidity EIA was used to detect antibody avidity among 117 treatment naïve HIV-1 infected individuals. A second-generation HIV ELISA was modified for in-house antibody avidity testing and cutoff was set based on Receiver Operating Characteristic (ROC) analysis. Archived paired samples from 25 HIV-1 infected individuals before ART and after successful ART; samples from 7 individuals responding to ART and during virological failure were also tested by LAg Avidity EIA.Results:Six individuals (5.1%) were identified as recently infected by a combination of LAg avidity assay and HIV-1 viral load testing. The modified in-house avidity assay demonstrated sensitivity and specificity of 100% and 98.2%, respectively, at AI=0.69 by ROC analysis. Median ODn values of individuals when responding to ART were significantly lower than pre-ART [4.136 (IQR 3.437– 4.827) vs 4.455 (IQR 3.748–5.120), p=0.006] whereas ODn values were higher during virological failure [4.260 (IQR 3.665 – 4.515) vs 2.868 (IQR 2.247 – 3.921), p=0.16].Conclusion:This modified in-house antibody avidity assay is an inexpensive method to detect recent HIV-1 infection. ART demonstrated significant effect on HIV-1 antibody avidity owing to changes in viral kinetics. |
| Databáze: | OpenAIRE |
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