Ectopic expression of NCAM in mouse fibroblasts stimulates self-aggregation, and promotes integration into primary cerebellum cell aggregates
| Autor: | Gaby Vopper, Ursula Hartmann, Conchita Vens, Wolfgang Wille, Uwe A.O. Heinlein |
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| Rok vydání: | 1994 |
| Předmět: |
Cerebellum
DNA Complementary Cell Adhesion Molecules Neuronal Cell Gene Expression Biology Transfection Exon Mice L Cells medicine Animals Neural cell Cells Cultured Cell Aggregation General Medicine Exons Fibroblasts Isotype Molecular biology medicine.anatomical_structure nervous system Ectopic expression Neural cell adhesion molecule |
| Zdroj: | Cell adhesion and communication. 2(4) |
| ISSN: | 1061-5385 |
| Popis: | We have undertaken aggregation experiments using mouse LMTK(-)-fibroblasts transfected with various isotypes of the neural cell adhesion molecule, NCAM. We found that self-aggregation of NCAM-positive fibroblasts is enhanced compared to control-transfected cells. The aggregation properties are partly dependent on the expressed NCAM isotype. Fibroblasts expressing a NCAM 140 isotype with exons a3 and pi were further tested in primary cerebellum cell re-aggregation experiments. While control-transfected fibroblasts could not be found in forming aggregates, fibroblasts ectopically expressing NCAM were integrated into neural cell aggregates. Time-lapse photography indicated that the nascent primary cell aggregates actively participated in the integration process by migration and attachment to nearby NCAM-positive fibroblasts. |
| Databáze: | OpenAIRE |
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