Effect of Poly(trehalose methacrylate) Molecular Weight and Concentration on the Stability and Viscosity of Insulin

Autor:	Madeline B. Gelb, Heather D. Maynard
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Materials science Polymers and Plastics General Chemical Engineering Insulin medicine.medical_treatment Organic Chemistry Polymer Methacrylate Trehalose Article Viscosity chemistry.chemical_compound chemistry Chemical engineering Materials Chemistry medicine
Zdroj:	Macromol Mater Eng
ISSN:	1439-2054 1438-7492
DOI:	10.1002/mame.202100197
Popis:	Instability to storage and shipping conditions and injection administration remain major challenges in treating chronic conditions with biopharmaceuticals. Herein, formulations of poly(trehalose methacrylate) (pTrMA) were successfully optimized to stabilize insulin without appreciably increasing viscosity. Polymers were synthesized (2,400 – 29,200 Da), and added to insulin at different concentrations. pTrMA maintained >95% intact insulin against 250 rpm at 37 °C for 3 hours with at least 10 mol. eq. of 5.0 kDa, 7.5 mol. eq. of 9.4 kDa, 5 mol. eq. of 12.8 kDa, 1 mol. eq. of 19.8 kDa, and 0.5 mol. eq. of 29.2 kDa polymers, compared to 13.1% of insulin alone. The lowest pTrMA concentration formulations were more viscous than insulin alone, but the highest viscosity, U-600 with 10 mol. eq. of 5 kDa pTrMA, was only 1.43 cP at 25 °C. This data demonstrates that pTrMA is a promising low viscosity additive to stabilize the diabetes therapeutic insulin.
Databáze:	OpenAIRE
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