Use of double labeling and photo CD for morphometric analysis of injured skeletal muscle
| Autor: | William T. Stauber, Gerald R. Miller, Martin J. Gibala, J. D. MacDougall |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Histology
Biopsy Physical Exertion Desmin Dystrophin Muscular Diseases Photography medicine Humans Myocyte Regenerating fibers Muscle Skeletal Compact Disks Double labeling medicine.diagnostic_test biology Chemistry Antibodies Monoclonal Skeletal muscle medicine.anatomical_structure Microscopy Fluorescence Morphometric analysis biology.protein Anatomy Biomedical engineering |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 43:1179-1184 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/43.11.7560901 |
| Popis: | We used computer-assisted analysis of myofiber cross-sectional areas to measure skeletal muscle responses to injury and disease. We developed a simple, inexpensive method for measuring myofiber size in human muscle samples using Kodak photo compact discs (CDs) as the image source. The photo CD serves as a permanent image storage medium and provides a high-resolution image that can be used to detect small myofibers. The use of double labeling for dystrophin and desmin allowed positive identification of both degenerating and regenerating fibers in a single biopsy specimen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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