Urease Color Test Medium U-9 for the Detection and Identification of 'T' Mycoplasmas in Clinical Material
| Autor: | Carl D. Lunceford, Maurice C. Shepard |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
food.ingredient
Urease medicine.drug_class Sodium Microorganism Antibiotics chemistry.chemical_element Biology Benzylpenicillin General Biochemistry Genetics and Molecular Biology Microbiology chemistry.chemical_compound food Amphotericin B medicine Agar General Pharmacology Toxicology and Pharmaceutics Bacteriological Techniques Chromatography General Immunology and Microbiology General Medicine Culture Media chemistry Urea biology.protein Clinical Microbiology Virology and Immunology medicine.drug |
| Zdroj: | Applied Microbiology. 20:539-543 |
| ISSN: | 0003-6919 |
| DOI: | 10.1128/am.20.4.539-543.1970 |
| Popis: | A urease color test fluid medium (U-9) for the detection and identification of T (T-strain) mycoplasmas in clinical material is described which is sensitive and specific for this group of mycoplasmas. The medium was prepared from commercially available components and contained 95% half-strength, tryptic digest broth ( p H 5.5), 4% unheated horse serum, 0.05% highest-purity urea, 0.001% sodium phenolsulfonphthalein, and 1,000 units of potassium penicillin G per ml. The final reaction of medium U-9 was p H 6.0. The overall agreement (positive and negative) between urease reactions in U-9 urease color test medium and culture findings in a standard agar primary culture system among 686 clinical specimens was 98.1%. The disagreement consisted of 13 false-positive urease reactions which were recognized visually as false-positive reactions due to other microorganisms. For specimens from the female genitourinary tract, the inclusion of 2.5 μg of amphotericin B (Fungizone) per ml of medium U-9 is recommended for the suppression of growth of Candida species and filamentous fungi. |
| Databáze: | OpenAIRE |
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