The Three Members of the Arabidopsis thaliana Glycosyltransferase Family 92 are Functional β-1,4-Galactan Synthases
| Autor: | Tomas Laursen, April J. M. Liwanag, Michela Catena, Antony Bacic, Emilie A. Rennie, Venkataramana R. Pidatala, William G.T. Willats, Mads Hartvig Clausen, Solomon Stonebloom, Jenny C. Mortimer, Joshua L. Heazlewood, Roshan Cheetamun, Mathias Christian Franch Andersen, Xiaoyuan Guo, Henrik Vibe Scheller, Carsten Rautengarten, Devon Birdseye, Berit Ebert, Pawel Gluza |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Mutant Arabidopsis Golgi Apparatus Plant Science Genes Plant 01 natural sciences Galactans Substrate Specificity Cell wall 03 medical and health sciences chemistry.chemical_compound Biosynthesis Cell Wall Glycosyltransferase Tobacco Arabidopsis thaliana biology Galactan Arabidopsis Proteins Glycosyltransferases food and beverages Cell Biology General Medicine biology.organism_classification Galactosyltransferases Pectin Recombinant Proteins Plant Leaves 030104 developmental biology chemistry Biochemistry Galactose biology.protein 010606 plant biology & botany Rhamnogalacturonan-I Subcellular Fractions |
| Zdroj: | Ebert, B; Birdseye, D; Liwanag, AJM; Laursen, T; Rennie, EA; Guo, X; et al.(2018). The Three Members of the Arabidopsis thaliana Glycosyltransferase Family 92 are Functional β-1,4-Galactan Synthases.. Plant & cell physiology. doi: 10.1093/pcp/pcy180. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/5v02w456 Ebert, B, Birdseye, D, Liwanag, A J M, Laursen, T, Rennie, E A, Guo, X, Catena, M, Rautengarten, C, Stonebloom, S H, Gluza, P, Pidatala, V, Andersen, M C F, Cheetamun, R, Mortimer, J C, Heazlewood, J L, Bacic, A, Clausen, M H, Willats, W G T & Scheller, H V 2018, ' The Three Members of the Arabidopsis thaliana Glycosyltransferase Family 92 are Functional β-1,4-Galactan Synthases ', Plant and Cell Physiology, vol. 59, no. 12, pp. 2624-2636 . https://doi.org/10.1093/pcp/pcy180 |
| DOI: | 10.1093/pcp/pcy180. |
| Popis: | Pectin is a major component of primary cell walls and performs a plethora of functions crucial for plant growth, development and plant-defense responses. Despite the importance of pectic polysaccharides their biosynthesis is poorly understood. Several genes have been implicated in pectin biosynthesis by mutant analysis, but biochemical activity has been shown for very few. We used reverse genetics and biochemical analysis to study members of Glycosyltransferase Family 92 (GT92) in Arabidopsis thaliana. Biochemical analysis gave detailed insight into the properties of GALS1 (Galactan synthase 1) and showed galactan synthase activity of GALS2 and GALS3. All proteins are responsible for adding galactose onto existing galactose residues attached to the rhamnogalacturonan-I (RG-I) backbone. Significant GALS activity was observed with galactopentaose as acceptor but longer acceptors are favored. Overexpression of the GALS proteins in Arabidopsis resulted in accumulation of unbranched β-1, 4-galactan. Plants in which all three genes were inactivated had no detectable β-1, 4-galactan, and surprisingly these plants exhibited no obvious developmental phenotypes under standard growth conditions. RG-I in the triple mutants retained branching indicating that the initial Gal substitutions on the RG-I backbone are added by enzymes different from GALS. |
| Databáze: | OpenAIRE |
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