Extracellular vesicle RNAs reflect placenta dysfunction and are a biomarker source for preterm labour
| Autor: | Leroy Hood, David Baxter, Taek-Kyun Kim, Kelsey Scherler, Xiaogang Wu, Kai Wang, Oksana Shynlova, Min Young Lee, Stephen J. Lye, Shannon Fallen |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Small RNA Placenta next‐generation sequencing Disease Bioinformatics preterm labour Exosome 03 medical and health sciences Extracellular Vesicles 0302 clinical medicine Obstetric Labor Premature Pregnancy microRNA Medicine exosome Chromosomes Human Humans Gene Regulatory Networks business.industry Sequence Analysis RNA RNA preterm birth Reproducibility of Results Cell Biology Extracellular vesicle Original Articles 3. Good health MicroRNAs 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis embryonic structures Molecular Medicine Biomarker (medicine) Original Article Female extracellular vesicle business Biomarkers |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | Preterm birth (PTB) can lead to lifelong complications and challenges. Identifying and monitoring molecular signals in easily accessible biological samples that can diagnose or predict the risk of preterm labour (PTL) in pregnant women will reduce or prevent PTBs. A number of studies identified putative biomarkers for PTL including protein, miRNA and hormones from various body fluids. However, biomarkers identified from these studies usually lack consistency and reproducibility. Extracellular vesicles (EVs) in circulation have gained significant interest in recent years as these vesicles may be involved in cell‐cell communication. We have used an improved small RNA library construction protocol and a newly developed size exclusion chromatography (SEC)‐based EV purification method to gain a comprehensive view of circulating RNA in plasma and its distribution by analysing RNAs in whole plasma and EV‐associated and EV‐depleted plasma. We identified a number of miRNAs in EVs that can be used as biomarkers for PTL, and these miRNAs may reflect the pathological changes of the placenta during the development of PTL. To our knowledge, this is the first study to report a comprehensive picture of circulating RNA, including RNA in whole plasma, EV and EV‐depleted plasma, in PTL and reveal the usefulness of EV‐associated RNAs in disease diagnosis. |
| Databáze: | OpenAIRE |
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