Immunohistochemical expression of β-catenin, Ki67, CD3 and CD18 in canine colorectal adenomas and adenocarcinomas
| Autor: | Gjermund Gunnes, Ellen Skancke, Runa Rørtveit, Linh T. Tran, Øyvor Kolbjørnsen, Kristin Marie Valand Herstad |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adenoma
Pathology medicine.medical_specialty CD3 Complex Markers for tumor progression 040301 veterinary sciences Colorectal cancer Colon Colorectal adenoma Adenocarcinoma Canine 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Immunophenotyping Dogs medicine Biomarkers Tumor Animals Tumor-infiltrating immune cells Dog Diseases beta Catenin Retrospective Studies Cell Nucleus Lamina propria lcsh:Veterinary medicine General Veterinary business.industry 04 agricultural and veterinary sciences General Medicine medicine.disease Immunohistochemistry digestive system diseases medicine.anatomical_structure Ki-67 Antigen Tumor progression 030220 oncology & carcinogenesis CD18 Antigens Case-Control Studies lcsh:SF600-1100 business Colorectal Neoplasms Colorectal adenoma and adenocarcinoma Research Article |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 17, Iss 1, Pp 1-9 (2021) |
| Popis: | Background Inflammation is believed to influence human colorectal carcinogenesis and may have an impact on prognosis and survival. The mucosal immunophenotype in dogs with colorectal cancer is poorly described. The aim of this study was to investigate whether the density, distribution and grade of tumor-infiltrating immune cells (TIIs) are different in normal colonic tissue vs benign stages (adenomas) and malignant stages (adenocarcinomas) of canine colorectal carcinogenesis, and thus, whether they can be considered as prognostic factors in dogs. This retrospective case-control study was performed on formalin-fixed, paraffin-embedded tissue samples from dogs with histologically confirmed colorectal adenoma (n = 18) and adenocarcinoma (n = 13) collected from archived samples. The samples had been collected by colonoscopy, surgery or during postmortem examination. Healthy colonic tissue obtained post mortem from dogs euthanized for reasons not involving the gastrointestinal tract served as control tissue (n = 9). Results The tumor samples had significantly lower numbers of CD3+ T-cells in the epithelium compared to controls (adenocarcinoma vs control, Kruskal-Wallis test, p = 0.0004, and adenoma vs control, p = 0.002). Adenomas had a significantly lower number of CD18+ cells in the lamina propria, compared to control samples (Kruskal-Wallis test, p = 0.008). Colonic samples from control dogs had uniform staining of β-catenin along the cell membrane of epithelial cells. Compared to normal colonic cells, the expression levels of cytoplasmic β-catenin were significantly higher in adenomas and adenocarcinomas (adenoma vs control Kruskal-Wallis test, p = 0.004, and adenocarcinoma vs control, p = 0.002). None of the control samples showed positive staining of β-catenin in the nucleus of colonic cells. In contrast, adenocarcinomas and adenomas showed moderate to strong staining of the cell nucleus. The nuclear β-catenin expression (signal strength and distribution) was significantly higher in adenomas compared to adenocarcinomas (Kruskal-Wallis test, p Conclusions β-catenin and Ki67 were not useful markers for demonstrating tumor progression from adenomas to adenocarcinomas. The lower presence of CD18 and CD3+ cells in colorectal tumors compared to controls indicates a reduced presence of histiocytes and T-cells, which may have implications for the pathogenesis and progression of colorectal cancer in dogs. |
| Databáze: | OpenAIRE |
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