Noma affected children from Niger have distinct oral microbial communities based on high-throughput sequencing of 16S rRNA gene fragments
| Autor: | Katrine Whiteson, Myriam Girard, Jacques Schrenzel, Heather Maughan, Patrice Francois, Manuela Tangomo-Bento, Vladimir Lazarevic, Didier Pittet |
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| Přispěvatelé: | Small, Pamela LC, Baratti, Denise, Pittet Cuenod, Brigitte Maud |
| Rok vydání: | 2014 |
| Předmět: |
Male
GESNOMA study group Noma Medical and Health Sciences RNA Ribosomal 16S Prevotella Medicine and Health Sciences 2.1 Biological and endogenous factors 2.2 Factors relating to the physical environment Niger Aetiology Child ddc:616 Pediatric biology Ecology ddc:617 lcsh:Public aspects of medicine Prevotella intermedia Bacterial Biological Sciences Peptostreptococcus Infectious Diseases Child Preschool Female medicine.symptom Infection Research Article DNA Bacterial 16S lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Oral Medicine Microbiology Lesion Tropical Medicine medicine Humans Dental/Oral and Craniofacial Disease Preschool Molecular Biology Nutrition Ribosomal Mouth Bacteria Prevention Public Health Environmental and Occupational Health Biology and Life Sciences lcsh:RA1-1270 Fusobacteria DNA biology.organism_classification medicine.disease Tropical Diseases Health Care Cross-Sectional Studies Fusobacterium Spirochaete RNA |
| Zdroj: | PLoS neglected tropical diseases, vol 8, iss 12 PLOS Neglected Tropical Diseases, Vol. 8, No 12 (2014) P. e3240 Whiteson, KL; Lazarevic, V; Tangomo-Bento, M; Girard, M; Maughan, H; Pittet, D; et al.(2014). Noma Affected Children from Niger Have Distinct Oral Microbial Communities Based on High-Throughput Sequencing of 16S rRNA Gene Fragments. PLoS Neglected Tropical Diseases, 8(12). doi: 10.1371/journal.pntd.0003240. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/8kk3294d PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Vol 8, Iss 12, p e3240 (2014) |
| ISSN: | 1935-2727 |
| DOI: | 10.1371/journal.pntd.0003240. |
| Popis: | We aim to understand the microbial ecology of noma (cancrum oris), a devastating ancient illness which causes severe facial disfigurement in>140,000 malnourished children every year. The cause of noma is still elusive. A chaotic mix of microbial infection, oral hygiene and weakened immune system likely contribute to the development of oral lesions. These lesions are a plausible entry point for unidentified microorganisms that trigger gangrenous facial infections. To catalog bacteria present in noma lesions and identify candidate noma-triggering organisms, we performed a cross-sectional sequencing study of 16S rRNA gene amplicons from sixty samples of gingival fluid from twelve healthy children, twelve children suffering from noma (lesion and healthy sites), and twelve children suffering from Acute Necrotizing Gingivitis (ANG) (lesion and healthy sites). Relative to healthy individuals, samples taken from lesions in diseased mouths were enriched with Spirochaetes and depleted for Proteobacteria. Samples taken from healthy sites of diseased mouths had proportions of Spirochaetes and Proteobacteria that were similar to healthy control individuals. Samples from noma mouths did not have a higher abundance of Fusobacterium, casting doubt on its role as a causative agent of noma. Microbial communities sampled from noma and ANG lesions were dominated by the same Prevotella intermedia OTU, which was much less abundant in healthy sites sampled from the same mouths. Multivariate analysis confirmed that bacterial communities in healthy and lesion sites were significantly different. Several OTUs in the Orders Erysipelotrichales, Clostridiales, Bacteroidales, and Spirochaetales were identified as indicators of noma, suggesting that one or more microbes within these Orders is associated with the development of noma lesions. Future studies should include longitudinal sampling of viral and microbial components of this community, before and early in noma lesion development. Author Summary Noma is a traumatic disease characterized by oral-facial lesions that often lead to severe disfigurement and ultimately shame and isolation from the community. Because the causes of noma are likely to be numerous, and reaching those who suffer from this illness is challenging, the etiology of noma remains ill-defined. Although it is known that oral hygiene and nutrition influence the development of noma, evidence suggests that one or more microbes play a crucial role in development of noma lesions. Previous studies have examined the DNA of microbes in lesions to determine which species are present and how their abundances differ between healthy mouth sites and noma lesions. These studies used techniques that were state-of-the-art at the time, though we know they likely only scratched the surface of the resident microbial diversity. Here we extend these studies by digging deeper to characterize a larger diversity of microbial species in noma and control samples, with the goal of better identifying which microbes are uniquely present or have altered abundances in noma lesions. |
| Databáze: | OpenAIRE |
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