Mixed-Mode Hydrophilic Interactions/Reversed-Phase Retention Mechanism in Thin-Layer Chromatography
| Autor: | Teresa Kowalska, Darija Obradović, Danica Agbaba |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Chromatography
Reverse-Phase Acetonitriles Chromatography Silica gel Methanol Hydrophilic interaction chromatography 010401 analytical chemistry Diol Silica Gel General Medicine 010402 general chemistry 01 natural sciences Thin-layer chromatography 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Phase (matter) Volume fraction Chromatography Thin Layer Solubility Acetonitrile Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of Chromatographic Science. 60:372-386 |
| ISSN: | 1945-239X 0021-9665 |
| Popis: | We investigated the dual retention mechanism in thin-layer chromatography taking place on three stationary phases of different polarity (C-18, plain silica gel and DIOL) and using binary mobile phases composed of acetonitrile as the main component and water, or methanol as a modifier. As the test analytes, we selected a set of 12 compounds of pharmaceutical importance and considerably different chemical structure, i.e. the imidazoline and serotonin receptor ligands, and their related compounds. Retention of each analyte in each investigated chromatographic system was determined in a wide enough range of the mobile phase composition, with volume fraction of the mobile phase modifier ranging from 0.10 to 0.90. Calculation of the exact turning point values as a proof of occurrence of the reversed-phase hydrophilic interaction chromatography (HILIC/RP) retention mechanism was based on the multimodal retention model. The dual retention mode was described with the use of the volume fraction of the mobile phase modifier, the total polarity and the total solubility models. For the DIOL, C-18 and silica gel stationary phase, the dual (HILIC/RP) retention mechanism was confirmed. In the case of the DIOL stationary phase and acetonitrile/methanol mobile phase, the observed retention mechanism was more complicated than the dual HILIC/RP one. |
| Databáze: | OpenAIRE |
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